Antibodies against nfatc1 and c fos

RAW264.7 cells were purchased from the American Type Culture Collection (Rockville, MD). Fetal bovine serum (FBS) and Alpha minimal essential Medium (α‐MEM) were obtained from Gibco (life technologies, CA). Penicillin‐streptomycin solution was obtained from Hyclone (Thermo Scientific, MA). Recombinant Mouse M‐CS F and Recombinant Mouse RANKL were obtained from R&D Systems (Minneapolis, MN). Cell Counting Kit‐8 was obtained from Dojindo Molecular Technologies (Dojindo, Japan). TRAP stain kit was obtained from Sigma–Aldrich (St. Louis, MO). Actin Cytoskeleton and Focal Adhesion Staining Kit was purchased from Millipore (Darmstadt, Germany). Antibodies against P38, p‐P38, ERK, p‐ERK, JNK, p‐JNK, and β‐actin were purchased from Cell Signaling (Danvers, MA). ERK, JNK, and P38 MAP kinase inhibitors were purchased from Medchemexpress (Princeton, NJ). Bovine cortical bone slices for Pit formation assay were obtained from Boineslices.com (Jelling, Denmark). Antibodies against NFATC1 and c‐FOS were purchased from Abcam (Cambridge). Staphylococcal protein A was purchased from Sigma–Aldrich.

DADS (purity >98%), TRAP Stain Kit, and LPS were purchased from MilliporeSigma (Burlington, MA, USA). DMEM, α minimal essential medium (α-MEM), and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Recombinant mouse M-CSF and recombinant mouse RANKL were obtained from R&D Systems (Minneapolis, MN, USA). Actin Cytoskeleton and Focal Adhesion (FAK) Staining Kit was purchased from Merck (Darmstadt, Germany). Osteo Assay Stripwell Plates for bone resorption were purchased from Corning (Corning, NY, USA). Antibodies against NFATc1 and c-Fos were purchased from Abcam (Cambridge, MA, USA). Specific antibodies against phosphorylated (p)-NFκB p65, NF-κB p65, p–IκB-α, IκB-α, p-STAT3 Ser727, p-STAT3 Tyr705, STAT3, and β-actin were obtained from Bioworld Technology (St. Louis Park, MN, USA). Mouse IL-1β, IL-6, and TNF-α ELISA Kit were obtained from Bioss (Beijing, China). Pierce Classic Magnetic Immunoprecipitation (IP)/Coimmunoprecipitation (Co-IP) Kit was purchased from Thermo Fisher Scientific.