Anti gapdh antibody ma5 15738

TG, pluronic F-127, and Fura-2 AM were purchased from Invitrogen. All chemicals for extracellular buffer (ECB; 125 mM NaCl, 5 mM KCl, 1.5 mM MgCl₂, 20 mM Hepes, 10 mM glucose, and 1.5 mM CaCl₂, pH 7.4), penicillin and streptomycin solutions, rapamycin, histamine, and EGTA were obtained from Sigma-Aldrich. siRNAs used in this study were generated as described previously (Liou et al., 2005 (link)). Primers used for siRNA generation are listed in Table S1. Monoclonal anti-PI(4,5)P₂ antibody (clone 2C11) and anti-PI(4)P antibody were obtained from Echelon Bioscience. Anti-RASSF4 antibody (C18182) was obtained from Assay Biotech. Anti-STIM1 antibody (4916) was obtained from Cell Signaling Technology. Anti-Orai1 antibody (PA1-74181) and anti-GAPDH antibody (MA5-15738) were obtained from Thermo Scientific. Anti-GFP antibody (ab290) was purchased from Abcam. Anti-mCherry antibody (632496) was purchased from Clontech. Protein standard (161-0373) and DNA ladder (SM1153) were purchased from Bio-Rad and Thermo Scientific, respectively.

The primary antibody against actin (SC-8432) was obtained from Santa Cruz Biotechnology (Santa Cruz, USA). The anti-alpha (α) tubulin antibody (T6199) was obtained from Sigma-Aldrich, while the anti-GAPDH antibody (MA5-15738) and the anti-acetyl-alpha (α) Tubulin (Lys40) antibody (32–2700) were obtained from Thermo Fisher Scientific (San Jose, USA). The secondary antibodies conjugated to HRP and goat-anti-rabbit IgG (NB7160) were obtained from Novus Biologicals (Littleton, USA), while Goat anti-mouse IgG (A-10677) was obtained from Thermo Fisher Scientific.