Luciferin luciferase based atp assay kit

Manufactured by Thermo Fisher Scientific

The Luciferin-luciferase based ATP assay kit is a laboratory tool used to measure the concentration of adenosine triphosphate (ATP) in a sample. The kit contains the necessary reagents, including luciferin and luciferase enzymes, to facilitate a bioluminescent reaction that quantifies the amount of ATP present.

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Lab products found in correlation

A1443001, by Thermo Fisher Scientific (1 mentions) Bca assay, by Thermo Fisher Scientific (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Cell Signaling Technology (1 mentions) Ripa buffer, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Luciferin/luciferase assay ATP assay kit Luciferase assay Luciferin

2 protocols using luciferin luciferase based atp assay kit

Quantifying Cellular ATP Under Nutrient Stress

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HCEC seeded at 1.5 × 10⁴/mL in 12-well plate were cultured to sub-confluent, then put under serum-free conditional DMEM medium (glucose free, glutamine-free, pyruvate-free, Gibco #A1443001) with glucose and/or glutamine supplementation for 12 h before measurement. ATP is extracted by a boiling water method (Yang et al., 2002 (link)), and measured by luciferin-luciferase based ATP assay kit (A22066, Molecular Probes).

Zhang W., Li H., Ogando D.G., Li S., Feng M., Price FW J.r., Tennessen J.M, & Bonanno J.A. (2017). Glutaminolysis is Essential for Energy Production and Ion Transport in Human Corneal Endothelium. EBioMedicine, 16, 292-301.

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ATP Quantification in MCEC Lysates

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MCEC after the incubation procedure mentioned above, were lysed in RIPA Buffer (#9806, Cell Signaling) plus PMSF (#8553, Cell Signaling) and ATP was immediately measured by luciferin-luciferase based ATP assay kit (A22066, Molecular Probes) in a microplate reader. Results were normalized to protein measured by BCA assay (#23225, Thermo Fisher Scientific).

Ogando D.G., Choi M., Shyam R., Li S, & Bonanno J.A. (2019). Ammonia sensitive SLC4A11 mitochondrial uncoupling reduces glutamine induced oxidative stress. Redox Biology, 26, 101260.

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