Anti total fak

Anti-α-actinin-4 was from Santa Cruz Biotechnology, Inc (Dallas, TX, USA, Cat #: SC-49333); anti-CD11b was from CedarLane Laboratories Limited (Hornby, Ontario, Canada, Cat #: CL8941AP); anti-Fibronectin was from Sigma (St. Louis, MO, USA, Cat #: F3648); anti-Integrin α8 was from R&D Systems (Minneapolis, MN, USA, Cat #: AF4076); anti-Laminin α1 was a gift from Dr. Dale Abrahamson (KU Medical Center, Kansas City, KS, rat monoclonal 8B3); anti-Laminin α2 and anti-β actin were from Sigma (St. Louis, MO, USA, Cat #: L0663); anti-Laminin α5 was a gift from Dr. Jeff Miner (Washington University, St. Louis, MO); anti-p-FAK³⁹⁷ was from Assay Biotechnology (Sunnyvale, CA, USA, Cat #: A0925) and from Invitrogen (Carlslab, CA); anti-Total FAK was from Cell Signaling Technology (Danvers, MA, USA, Cat #: 3285). Anti-MMP-10 antibodies were from Millipore (Billerica, MA, USA, Cat # ABT 289). All Alexa-fluor conjugated secondary antibodies were from Invitrogen (Carlsbad, CA), including donkey anti-rat 488, donkey anti-rabbit 555, goat anti-rat 488, goat anti-rabbit 555, donkey anti-rabbit 488, and donkey anti-goat 568. The small molecular inhibitor for FAK activation, TAE226 was from Chem Scene (Monmouth Junction, NJ, Cat #CS-0594); the peptide inhibitor for NF-kappaB (SN-50) was from Calbiochem (now EMD Millipore, Billerica, MA, Cat #481480)

Antibodies were purchased: monoclonal antibodies, anti-integrin αvβ3, anti-integrin β3(CD61), and anti-integrin α5β1 (Millipore, Billerica, MA), anti-human CD171 (L1CAM; BD Pharmingen, San Diego, CA), anti-integrin αvβ5 (R&D Systems, Minneapolis, MN), anti-actin, anti-β-tubulin, anti-phospho-ERK, and anti-phospho-JNK (Santa Cruz, Dallas, TX), and anti-L1CAM UJ127 (GeneTex, Irvine, CA); rabbit antibodies, anti-integrin β3 (Epitomics, Burlingame, CA), and anti-phospho-FAK (Y397), anti-total FAK, anti-phospho-BMX/Etk (Y40), anti-total BMX/Etk, and anti-total p130CAS (E1L9H) (Cell Signaling, Beverly, MA). Rabbit affinity purified phospho-specific anti-p130CAS (Y234) was generated using the following peptide: -AQPEQDE[pY]DIPRHL, corresponding to residues 227-240 in human p130CAS, by 21st Century Biochemicals, Inc (Marlboro, MA) (SFigure 4).