Goat anti mouse igg hrp polyclonal antibody

Fetal bovine serum (FBS), fibroblast growth factor, heparin and trypsin-EDTA were procured from Invitrogen Bioservices, India. Linalool, monoclonal antibodies such as p-ERK1, p-JNK, p-p38, IκBα, NF-κB, TNF-α, IL-6, IL-10, COX-2, Bax, Bcl-2, p53, MMP-2, MMP-9,β-actin, lamin-A and goat anti-mouse IgG-HRP polyclonal antibody, 3-(4, 5-dimethyl-2-thiaozolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT), 2,7-diacetyl dichlorofluorescein (DCFH-DA) were purchased from Sigma chemical Co., St. Louis, MO, USA.

Ursolic acid (purity ≥ 98%), thiobarbituric acid (TBA), phenazinemethosulphate (PMS), nitroblue tetrazolium (NBT), 5,5-dithiobis 2-nitrobenzoic acid (DTNB), 3-(4, 5-dimethyl-2-thiaozolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT), 2′7′-diacetyl dichlorofluorescein (DCFH-DA), rhodamine-123, and nicotinamide adenine dinucleotide (NAD) were supplied by Sigma-Aldrich, St. Louis, USA. The mouse monoclonal antibodies anti-TNF-α, anti-NF-κB (p65), anti-p53, anti-Bax, anti-Bcl-2, anti-caspase-3, anti-MMP-2, anti-MMP-9, anti-β-actin, and goat anti-mouse IgG-HRP polyclonal antibody were also purchased from Sigma-Aldrich, St. Louis, USA. RNA isolation kit, cDNA synthesis kit, PCR primers, and PCR master mix were purchased from Qiagen, USA. Bovine serum albumin (BSA), RIPA buffer, low melting agarose (LMPA), normal melting agarose (NMPA), phosphate buffered saline (PBS) and reduced glutathione (GSH) were purchased from Merck, India. All other chemicals, gradient solvents, and other analytical grades were acquired from S.D Fine Chemical and Himedia, India.