Streptavidin alexa 488 conjugate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Streptavidin-Alexa 488 conjugate is a fluorescently labeled streptavidin protein. Streptavidin binds strongly to biotin, a small molecule commonly used for labeling and detection in biological applications. The Alexa 488 fluorophore attached to the streptavidin allows for visualization and detection of biotinylated targets.

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Lab products found in correlation

Lsm 880, by Zeiss (1 mentions) Dmr microscope, by Leica (1 mentions) Cs 56, by Merck Group (1 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) Alexa 594 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti mouse igg alexa594, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg alexa488, by Thermo Fisher Scientific (1 mentions) C8035, by Merck Group (1 mentions)

Spelling variants of this product

Alexa 488 (1863 citations) Streptavidin-Alexa 488 (88 citations) Streptavidin-Alexa Fluor 488 conjugate (64 citations) Streptavidin-488 (24 citations) Streptavidin 488 conjugate (4 citations)

2 protocols using streptavidin alexa 488 conjugate

Fluorescence and DAB Labeling of Neurons

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After electrophysiological recordings with pharmacological protocols, the slices were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M phosphate buffer for 24 h, embedded in 6% gelatine then re-sectioned at 70 μm. For fluorescence labelling, the sections were permeated using 0.1% Triton X-100 and incubated with Streptavidin-Alexa 488 conjugate (Thermofisher, United States ) for 48 h followed by image acquisition, via confocal laser scanning microscope (LSM 880 Zeiss, Germany). After image acquisition the sections were washed and incubated in avidin-biotin complex (ABC) overnight at 4°C, followed by the 3-3-diaminobenzidine (DAB) staining. Recovered cells were reconstructed manually from consecutive slices at ×100 objective under a Leica DMR microscope with an attached drawing tube.

Aldabbagh Y., Islam A., Zhang W., Whiting P, & Ali A.B. (2022). Alzheimer’s Disease Enhanced Tonic Inhibition is Correlated With Upregulated Astrocyte GABA Transporter-3/4 in a Knock-In APP Mouse Model. Frontiers in Pharmacology, 13, 822499.

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Whole-mount Immunofluorescence Staining Protocol

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Whole-mount immunofluorescence was performed as previously described (Alexander et al., 1999 (link); Peal et al., 2009 (link)), using rhodamine phalloidin (Invitrogen R415) and the primary antibodies CS-56 (anti-chondroitin sulfate; C8035; Sigma; 1:200), Zn-5 (anti-Dm-grasp; Zebrafish International Resource Center; 1:10), A11122 (anti-GFP; Invitrogen; 1:100), MF20 (Developmental Studies Hybridoma Bank; 1:10), and F59 (Developmental Studies Hybridoma Bank; 1:10). Secondary antibodies used were anti-mouse IgG-Alexa 488 (A11029; Invitrogen; 1:100), anti-mouse IgG-Alexa 594 (A11005; Invitrogen; 1:100), anti-mouse IgG2b-TRITC (1090-03; Southern Biotech; 1:100), anti-rabbit IgG-Alexa 488 (A11008; Invitrogen; 1:100), and anti-rabbit IgG-Alexa 594 (A11012; Molecular Probes; 1:400). For visualization of HA localization, staining was performed as previously described (Tong et al., 2014 (link)), using biotinylated hyaluronan-binding protein (HABP) (EMD Millipore; 1:10) and a streptavidin-Alexa 488 conjugate (Thermo Fisher Scientific; 1:500).

Hernandez L., Ryckebüsch L., Wang C., Ling R, & Yelon D. (2019). Tmem2 restricts atrioventricular canal differentiation by regulating degradation of hyaluronic acid. Developmental dynamics : an official publication of the American Association of Anatomists, 248(12), 1195-1210.

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