K bzip

Western blotting was performed as previously described^{27 (link)} using primary antibodies against LANA (Abcam), K-bZIP (Santa Cruz), β-actin (Santa Cruz), XPO1 (Sigma), p62 (Cell Signaling), pTBK1 (Cell Signaling), TBK1 (Novus Biologicals), pIRF3 (Novus), IRF3 (Novus), STING (Novus), and LC3B (Sigma). ORF65 and RTA antibodies were previously described^{5 (link),28 (link)}.

Cells were collected in PBS and lysed in PBS containing 0.5% Nonidet P-40 (NP-40) containing 1X protease inhibitor cocktail (Roche, 4693132001). The cell lysates were sonicated 5 times (pulsed for 30 sec with 30 sec interval) using a Bioruptor (Diagenode, UCD-200) at low power setting. Total cell lysate (TCL) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene fluoride membranes (PVDF; PerkinElmer, 1002001), blocked with 5% nonfat skim milk or 5% BSA (UniRegion, UR-BSA001-50G) in 1X TBST, and probed with primary antibody at 4 ^oC for 16 hours. After incubating the membrane with horseradish peroxidase (HRP) linked secondary antibody (GE healthcare, NA934 and NA931), the protein expression levels were detected by Pierce ECL Western Blotting Substrate (Thermo, 32106). Primary antibodies against KDM4A (Polyclonal antibody purified from rabbit) [12 (link)], K-bZIP (1:1000; Santa Cruz Biotechnology, sc-69797), Orf45 (1:1000; Santa Cruz Biotechnology, sc-53883), HA tag (1:4000; Cell Signaling Technology, #3724) and α-Tubulin (1:4000; Sigma, T6074-200UL) were used in this study.