Cells were labelled with primary OV6 antibody (mouse IgG1; R&D Systems, Minneapolis), magnetically tethered to rat anti-mouse IgG1 microbeads, and sorted with a Mini-MACS™ Cell Sorter Kit (Miltenyi Biotec, CA). All of the procedures were following the manufacturer’s instructions. The sorted cells were evaluated by flow cytometry analysis. The flow cytometry was performed with MoFlo Sorter (Beckman, Brea, CA) using an APC-conjugated-OV6 antibody (R&D Systems, Minneapolis) and following manufacturer’s instruction.
Apc conjugated ov6 antibody
Manufactured by R&D Systems
Sourced in United States
The APC-conjugated-OV6 antibody is an immunochemical tool for cellular analysis. It is a conjugate of an allophycocyanin (APC) fluorescent dye and an antibody specifically targeting the OV6 antigen. This antibody can be used to detect and quantify OV6-expressing cells through flow cytometry or other immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Primary ov6 antibody mouse igg1, by R&D Systems (2 mentions)
Mini macs cell sorter kit, by Miltenyi Biotec (2 mentions)
Moflo sorter, by Beckman Coulter (2 mentions)
Bv421 conjugated anti cd34 antibody clone 581, by BD (2 mentions)
Facscanto 2, by BD (1 mentions)
Fitc conjugated anti cd90 antibody clone 5e10, by BioLegend (1 mentions)
Pe conjugated anti vimentin antibody clone d21h3, by Cell Signaling Technology (1 mentions)
Imagestreamx, by Merck Group (1 mentions)
Aria fusion cytometer, by BD (1 mentions)
Apc mouse igg1 isotype control, by BioLegend (1 mentions)
4 protocols using apc conjugated ov6 antibody
1
Enrichment and Characterization of OV6+ Cells
2
Multimarker Phenotypic Characterization of Cells
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The cells were stained for the surface expression of CD34 using a BV421-conjugated anti-CD34 antibody (clone 581, BD Horizon, Franklin Lakes, NJ, USA), the expression of CD90 using an FITC-conjugated anti-CD90 antibody (clone 5E10, BioLegend, Koblenz, Germany), the expression of vimentin using a PE-conjugated anti-vimentin antibody (clone D21H3, Cell Signaling Technology, Frankfurt, Germany) and the expression of CXCR4 using a PE-conjugated anti-CXCR4 antibody (clone12G5, R&D Systems, Biotechne, Wiesbaden, Germany). The cells were also stained with an APC-conjugated OV-6 antibody (R&D Systems, Biotechne, Wiesbaden, Germany), Data were acquired on a BD FACSCanto II (BD Biosciences, San Jose, CA, USA) and analyzed using the Flowjo software v7.2.5 (BD Life Sciences, Ashland, OR, USA).
3
Magnetically Sorted OV6+ Cells
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Cells were labelled with primary OV6 antibody (mouse IgG1; R&D Systems, Minneapolis), magnetically tethered to rat anti-mouse IgG1 microbeads, and sorted with a Mini-MACS™ Cell Sorter Kit (Miltenyi Biotec, CA). All of the procedures were following the manufacturer’s instructions. The sorted cells were evaluated by flow cytometry analysis. The flow cytometry was performed with MoFlo Sorter (Beckman, CA) or ImageStreamx (Millipore, US) using an APC-conjugated-OV6 antibody (R&D Systems, Minneapolis) and following manufacturer’s instruction.
4
Sorting of HuH6 Cells by CD34 and OV-6
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The HuH6 cells were simultaneously stained with a BV421-conjugated anti-CD34 antibody (clone 581, BD Horizon, Franklin Lakes, NJ, USA) and an APC-conjugated OV-6 antibody (R&D Systems, Biotechne, Wiesbaden, Germany). The CD34 and OV-6 double-negative cells were sorted in a BD Aria Fusion cytometer. The sorting gates were established using cells stained with isotype controls (Brilliant Violet 421 mouse IgG1 isotype control and APC mouse IgG1 isotype control, Biolegend, Koblenz, Germany). After the sorting, the collected CD34−OV-6− and CD34+OV-6+ cells were re-analyzed for CD34 expression and OV-6 binding using the cytometer.
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