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2 protocols using standards of catechin

1

Quantification of Cranberry Polyphenols

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Standards of catechin (CAT), epicatechin (EC), myricetin-3-O-glucoside, quercetin-3-O-glucoside, quercetin, procyanidin C1 (PC1), and procyanidin A2 (PA2) were purchased from Extrasynthese (Genay, France). Methanol, acetonitrile, 4-dimethylammino-cinnamaldehyde (DMAC), and acetic acid were from Sigma-Aldrich (St. Louis, MO, USA). Amicon ultra-4 centrifugal filter units of 3, 10, 30, 50, and 100 nominal molecular weight limits (NMWL) were supplied from Merck Millipore (Milan, Italy). Water was from a Milli-Q apparatus (Millipore, Milford, CT, USA). Commercial dried cranberry extracts (A1, A2, and A3) were obtained from different manufacturers. Notably, A1 and A3 were obtained from an industrial producer of natural ingredients starting from whole berry. On the contrary, A2 was produced by a small company through a proprietary purification and concentration process starting from cranberry commercial extracts. Details regarding the plant origins and manufacturing processes of the extracts are not available.
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2

Quantification of Polyphenolic Compounds

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Standards of catechin, epicatechin, procyanidin B1, procyanidin B2, delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, petunidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, quercetin 3-O-glucuronide, quercetin 3-O-glucoside, quercetin, kaempferol, chlorogeinc acid and phloretin, were purchased from Extrasynthese (Lyon, France). The integrated total dietary fibre assay procedure kit was purchased from Megazyme International Ireland Ltd. (Bray, Ireland) . All other chemicals were purchased from Sigma-Aldrich Italia (Milan, Italy).
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