Mda assay kit

50 mg of the left gastrocnemius muscle was homogenized and centrifuged for 10 minutes at 1,200 rpm while being preserved in 0.1 mol phosphate buffer saline (PBS) solution. The supernatant was used to determine the tissue levels of Malondialdehyde (MDA), Reduced Glutathione (GSH) and Superoxide dismutase (SOD).
Malondialdehyde (MDA) concentration in muscle tissue homogenate was measured colorimetrically using a lipid peroxidation (MDA) assay kit (Biodiagnostic, Giza, Egypt), according to the manufacturer’s instructions.
Utilizing the reduced glutathione colorimetric kit (Biodiagnostic, Giza, Egypt), reduced glutathione (GSH) content was determined in line with the manufacturer’s guidelines.
Superoxide dismutase (SOD) activity in muscle tissue was measured by Superoxide Dismutase Activity Colorimetric Assay Kit (Abcam, Catalog No. ab65354) following the manufacturer’s instructions.

The degree of myocardial lipid peroxidation was determined by measuring the level of MDA using an MDA assay kit (Biodiagnostic, Cairo, Egypt). According to the manufacturer’s protocol, a pink color is produced by the reaction of thiobarbituric acid with MDA. The absorbance was measured at 534 nm using a spectrophotometer (Analytik Jena^®, Jena, Germany) (Ohkawa et al., 1979 (link)).

We assessed various parameters, including lipid peroxidation, which was quantified using malondialdehyde (MDA), a major thiobarbituric acid reactive species. Additionally, we assessed carbonyl content, reduced glutathione (GSH), total antioxidant capacities (TAC), Myeloperoxidase (MPO) as well as catalase and superoxide dismutase (SOD) activities. These assessments were carried out in accordance with the manufacturer’s instructions, employing commercially available kits as follows: MDA assay kit (Biodiagnostics, Egypt, Cat # MD2529), Protein Carbonyl Colorimetric Assay Kit (Cayman, Ann Arbor, Michigan, United States, Cat # 10005020), GSH assay kit (Biodiagnostics, Egypt, Cat # GR2511), TAC assay kit (Biodiagnostics, Egypt, Cat # TA2513), Myeloperoxidase (Abcam, Cambridge, United Kingdom, Cat # ab105136), Catalase activity assay kit (Biodiagnostics, Egypt, Cat # SD2521), and SOD activity assay kit (Biodiagnostics, Egypt, Cat # CA2517).