6490 triple quad lc ms

Manufactured by Agilent Technologies

Sourced in United States

The 6490 Triple Quad LC/MS is a liquid chromatography-tandem mass spectrometry (LC/MS/MS) system designed for high-performance quantitative analysis. It features a triple quadrupole mass analyzer, providing selective and sensitive detection of target analytes in complex samples.

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Lab products found in correlation

1290 infinity hplc, by Agilent Technologies (2 mentions) Xselect hss t3 column, by Waters Corporation (2 mentions) 1290 infinity uv detector, by Agilent Technologies (2 mentions) 6490 triple quad mass detector, by Agilent Technologies (2 mentions) 1290 infinity, by Agilent Technologies (2 mentions) Masshunter quantitative analysis software, by Agilent Technologies (1 mentions) Ifunnel technology, by Agilent Technologies (1 mentions) Mass hunter software version b 06, by Agilent Technologies (1 mentions)

Close spelling variants of this product

6490 Triple Quad LC/MS System (6 citations) Agilent 6490 Triple Quad LC/MS (5 citations) 6490 Triple Quad (4 citations) 6490 Triple Quad MS (3 citations)

8 protocols using 6490 triple quad lc ms

LC-MS/MS Analysis of PIT Constituents

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LC-MS/MS technique has been used for identifying the main chemical constituents of PIT as previously described by Kongkiatpaiboon et al. [23 (link)]. The combination of chromatographic separation of LC-MS/MS system was combined by Agilent HPLC 1290 Infinity and mass analyzer 6490 Triple Quad LC/MS Agilent Technologies, which equipped with an electrospray ionization (ESI) source system. Agilent ZORBAX Rapid Resolution High Definition (RRHD) SB-C18, 2.1 mm id × 150 mm (1.8 μm) was used for chromatographic separation. The mobile phase system used 1% formic acid in water as solvent A and 1% formic acid in acetonitrile as solvent B. The gradient of the mobile phase was set at a ratio of solvent A : solvent B, 100 : 0, with gradient elution, from 30% solvent B at 10 min and 100% solvent B at 30 min at a flow-rate of 0.2 ml/min. The sample injection volume was 5 μL, and the column was set at 25°C. 4-CQ, 5-CQ, 3,4-CQ, 3,5-CQ, and 4,5-CQ were used as standard.

Sirichaiwetchakoon K., Lowe G.M, & Eumkeb G. (2020). The Free Radical Scavenging and Anti-Isolated Human LDL Oxidation Activities of Pluchea indica (L.) Less. Tea Compared to Green Tea (Camellia sinensis). BioMed Research International, 2020, 4183643.

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LC-MS/MS Profiling of Bioactive Compounds in B. superba

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The LC–MS/MS technique was employed to identify the chemical constituents present in B. superba. The combination of chromatographic separation in the LC–MS/MS system was combined from an Agilent HPLC 1290 Infinity with a mass analyzer 6490 Triple Quad LC/MS from Agilent Technologies, equipped with an electrospray ionization (ESI) source system. The setup consisted of an auto-sampler, a binary pump, and a vacuum degasser. An Agilent ZORBAX Rapid Resolution High Definition (RRHD) SB-C18, 2.1 mm id × 150 mm (1.8 µm) was used for chromatographic separation. The mobile phase system involved two solvents: solvent A, comprising 13 mM ammonium acetate buffer adjusted to pH 4 with 0.1% acetic acid, and solvent B, consisting of 0.1% acetic acid in methanol. The gradient elution method employed a ratio of solvent A to solvent B starting at 100:0, which gradually shifted to 60% solvent B at 5 min and reached 100% solvent B at 30 min, operating at a flow rate of 0.25 ml/min. The sample injection volume was set at 20 µl, and the column temperature was maintained at 40 °C throughout the analysis. Genistein, daidzein, and biochanin A were used as standard.

Sirichaiwetchakoon K, & Eumkeb G. (2024). Free radical scavenging and anti-isolated human LDL oxidation activities of Butea superba Roxb. extract. BMC Complementary Medicine and Therapies, 24, 75.

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LC-MS/MS Analysis of Pesticides

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An Agilent UPLC 1290 Series (Agilent Technologies, Palo Alto, CA, USA) coupled to an Agilent Technologies 6490 TripleQuad LC/MS was used for this study. The UPLC was equipped with a reversed-phase C8 analytical column of 2.1 mm × 100 mm and 1.8 mm particle size (Agilent Technologies, Santa Clara, CA). Gradient LC elution was performed with 0.1% formic acid, 5 mM ammonium formate and 2% ultrapure water in methanol as mobile phase A and 0.1% formic acid, 5 mM ammonium formate and 2% methanol in ultrapure water as mobile phase B. The mobile phase composition is as follows: 20% A (2 min), 13 min linear gradient to 100% A (3 min), 2.5 min post-run time back to the initial conditions. The flow rate was 0.3 mL min⁻¹ and the injection volume 5 µL. Analytical parameters of the mass spectrometer are published in a previous work (Gil García, Martínez Galera, Uclés, Lozano, & Fernández-Alba, 2018 (link)). Table S1 shows the acquisition parameters of the analyzed pesticides by LC-QqQ-MS/MS (see supplementary material).

Gómez-Ramos M.D., Nannou C., Martínez Bueno M.J., Goday A., Murcia-Morales M., Ferrer C, & Fernández-Alba A.R. (2020). Pesticide residues evaluation of organic crops. A critical appraisal. Food Chemistry: X, 5, 100079.

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Measuring Global DNA Methylation and Hydroxymethylation

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Global cytosine methylation and hydroxymethylation levels were determined using established HPLC protocols (Ramsahoye, 2002 (link)) with some modifications. Briefly, genomic DNA was hydrolyzed with a combination of RNase A and RNase T1 followed by ethanol precipitation. DNA was digested to nucleosides as described previously (Hashimoto et al., 2014 (link)) and separated on a 6490 Triple Quad LC-MS with UV absorbance detector (1290 Infinity UV detector, 6490 Triple Quad Mass detector, Agilent, Santa Clara, CA) equipped with an XSelect™ HSS T3 column (2.1 × 100mm, 2.5 μm, Waters, Milford, MA).

Aluru N., Kuo E., Helfrich L.W., Karchner S.I., Linney E.A., Pais J.E, & Franks D.G. (2015). Developmental exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin alters DNA methyltransferase (dnmt) expression in zebrafish (Danio rerio). Toxicology and applied pharmacology, 284(2), 142-151.

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Quantitative Analysis of Nucleotide Sugars

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Protein extracts were dissolved in 100 μL MilliQ water. Nucleotide sugars were analyzed by reverse‐phase ion pairing chromatography (Agilent Technologies 1290 Infinity) coupled to a triple quadrupole mass spectrometer operating in negative ion mode (Agilent Technologies 6490 Triple Quad LC‐MS), as previously described.¹⁴, ¹⁵ The following nucleotide sugars were detected in this analysis: UDP‐glucose (Glc), UDP‐galactose (Gal), UDP‐N‐acetylhexosamines (UDP‐HexNAc, composed of UDP‐N‐acetylglucosamine (UDP‐GlcNAc) and UDP‐N‐acetylgalactosamine (GalNAc)), CMP‐N‐acetylneuraminic acid (Neu5Ac), CMP‐N‐glycolylneuraminic acid (Neu5Gc), GDP‐fucose (Fuc), UDP‐glucuronic acid (GlcA), UDP‐xylose (Xyl), CDP‐ribitol, and ADP‐ribose. Peak integration of compounds was done using the Agilent MassHunter Quantitative Analysis software (version B.08.00); all peaks with a signal ratio lower than five were excluded from further analysis. Peak areas from each nucleotide sugar were normalized on the total metabolite peak area, and fold changes to controls were calculated. The WT profiles were compared to galt KO profiles. All analyses and calculations for individual nucleotide sugars were performed on natural log transformed data of the relative abundance.

Haskovic M., Coelho A.I., Lindhout M., Zijlstra F., Veizaj R., Vos R., Vanoevelen J.M., Bierau J., Lefeber D.J, & Rubio‐Gozalbo M.E. (2020). Nucleotide sugar profiles throughout development in wildtype and galt knockout zebrafish. Journal of Inherited Metabolic Disease, 43(5), 994-1001.

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Quantification of Phytochemicals in Medicinal Plant Extracts

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The total alkaloid, flavonoid, and polyphenol contents were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) operated with Agilent Technologies 6490 Triple Quad LC/MS coupled with Agilent Technologies 1290 Infinity. The total alkaloid, flavonoid, and polyphenol contents were carried out, with the following setting: Xiao et al. [13 (link)] for total alkaloid content and Sanchez-Rabaneda et al. [14 (link)] for total flavonoid and polyphenol contents. Rotundine (only the alkaloid found in rhizomes of C. rotundus [15 (link), 16 (link)]), quercetin, and gallic acid were employed as standard alkaloid, flavonoid, and polyphenol, respectively. The total phytochemical contents were calculated as follows:

\begin{matrix} T o t a l a l k a l o i d c o n t e n t (T A C) = \frac{R E \times V}{m} \\ T o t a l f l a v o n o i d c o n t e n t (T F C) = \frac{Q E \times V}{m} \\ T o t a l p o l y p h e n o l c o n t e n t (T P C) = \frac{G A E \times V}{m}, \end{matrix}

where V is the volume of extract (ml); m is the weight of dry extract (g); RE is a rotundine equivalent (mg/ml); QE is a quercetin equivalent (mg/ml); GAE is gallic acid equivalent (mg/ml).

Cheypratub P., Leeanansaksiri W, & Eumkeb G. (2018). The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin-Resistant Staphylococcus aureus. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 3438453.

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Quantifying Global Cytosine Levels

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Global cytosine levels were determined using established high performance liquid chromatography (HPLC) protocols (Ramsahoye, 2002 (link)) with some modifications. Briefly, genomic DNA was hydrolyzed with a combination of RNase A and RNase T1 followed by ethanol precipitation. DNA was digested to nucleosides as described previously (Hashimoto et al., 2015 (link)) and separated on a 6490 Triple Quad LC‐MS with UV absorbance detector (1290 Infinity UV detector, 6490 Triple Quad Mass detector, Agilent, Santa Clara, CA) equipped with an XSelect™ HSS T3 column (2.1 × 100 mm, 2.5 μm, Waters, Milford, MA).

Aluru N., Fields D.M., Shema S., Skiftesvik A.B, & Browman H.I. (2021). Gene expression and epigenetic responses of the marine Cladoceran, Evadne nordmanni, and the copepod, Acartia clausi, to elevated CO2. Ecology and Evolution, 11(23), 16776-16785.

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Lipidomic Analysis of Native LDL

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The lipids were extracted 49 from native LDL and isolated extracellular lipoprotein particles, dried under nitrogen flow, and dissolved in chloroform/methanol (1:2). The samples were spiked with a mixture of several quantitative standards, and just before the analysis, 1% (v/v) of NH 3 was added to each sample to support ionization and prevent adduct formation. The mass spectra, indicating all positive ions, all negative ions, or precursors of the ion m/z 184 (a specific fragment for the choline head group), were recorded by an Agilent 6490 Triple Quad LC/MS with iFunnel technology (Agilent Technologies, Santa Clara, CA). The data were further analyzed with MassHunter software version B.06.00, November 2012 (Agilent Technologies) and LIMSA (Somerharju Group, University of Helsinki, Helsinki, Finland) 50 software version 1.0 (Helsinki, Finland), the latter using the standards and a lipid library.

Lehti S., Nguyen S.D., Belevich I., Vihinen H., Heikkilä H.M., Soliymani R., Käkelä R., Saksi J., Jauhiainen M., Grabowski G.A., Kummu O., Hörkkö S., Baumann M., Lindsberg P.J., Jokitalo E., Kovanen P.T, & Öörni K. (2018). Extracellular Lipids Accumulate in Human Carotid Arteries as Distinct Three-Dimensional Structures and Have Proinflammatory Properties. The American journal of pathology, 188(2).

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