Madopar, which is a combination of levodopa and benserazide, was obtained from Shanghai Roche Pharmaceuticals Ltd. (Shanghai, China). Dopamine, DOPAC, HVA, 5-HT, NE, HIAA, and MPTP were purchased from Sigma-Aldrich (St. Louis, MO, USA). A bicinchoninic acid (BCA) protein determination kit was obtained from CoWin Biosciences, Beijing, China. Diaminobenzidine (DAB) staining, and Nissl staining kits were purchased from Beyotime (Nanjing, China). The Polyvinylidene fluoride (PVDF) transfer membrane was purchased from Millipore Corp. (Bedford, MA, USA). Primary antibodies were obtained from Affinity Biosciences, Cell Signal Transduction, Cincinnati, OH, USA. Table 2 shows the primary antibody specifications and dilutions.
Nissl staining kit
Manufactured by Beyotime
Sourced in China
The Nissl staining kit is a laboratory tool used to visualize nerve cells and their structures within tissue samples. It provides a simple and effective method for staining Nissl bodies, which are distinctive granular structures found in the cytoplasm of neurons. The kit includes all the necessary reagents and protocols to perform the Nissl staining process.
Automatically generated - may contain errors
Lab products found in correlation
Dopamine, by Merck Group (1 mentions)
Dopac, by Merck Group (1 mentions)
Polyvinylidene fluoride pvdf transfer membrane, by Merck Group (1 mentions)
Madopar, by Roche (1 mentions)
Enhanced chemiluminescence kit, by Beyotime (1 mentions)
Epigallocatechin gallate (egcg), by Yuanye Bio-Technology (1 mentions)
Camkii, by Proteintech (1 mentions)
Dab kit, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Dcfh da, by Beyotime (1 mentions)
Fluo 3 am, by Beyotime (1 mentions)
Beclin 1, by Proteintech (1 mentions)
Malondialdehyde mda kit, by Nanjing Jiancheng (1 mentions)
Vs120 microscope, by Olympus (1 mentions)
B 600 microscope, by Olympus (1 mentions)
Spot fiex camera, by Olympus (1 mentions)
Phosphate buffered saline (pbs), by ZSGB-BIO (1 mentions)
Dmi4000b, by Leica (1 mentions)
He staining kit, by Beyotime (1 mentions)
9 protocols using nissl staining kit
1
Evaluating Levodopa and Benserazide Combination
2
PLGA-based Neuroprotective Formulation
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mPEG-poly lactic-co-glycolic acid (PLGA) (75:25, 45,000) was purchased from Daigang Biomaterial Co., Ltd (Jinan, Shandong, China). EGCG (purity > 98%; Yuanye Biotechnology Co., Ltd, Shanghai, China) was dissolved in water (pH 3.0) and stored at −20°C. Nimodipine was obtained from Ruiyang Pharmaceutical Co., Ltd (Yi Yuan, Shandong, China). Lactate dehydrogenase (LDH), glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) kits were obtained from the Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China). JC-1, Fluo-3 AM, DCFH-DA, Nissl staining kits, and enhanced chemiluminescence kits were purchased from Beyotime (Shanghai, China). CaMKII, Atg5, Beclin-1, and Mn-SOD antibodies were obtained from Proteintech Group, Inc (Rosemont, IL, USA). SP-9002 SPlink detection and DAB kits were purchased from Zhongshan Golden Bridge Biotechnology (Beijing, China).
3
Nissl Staining of Mouse Brain Sections
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Mouse brain sections were stained using the Nissl Staining Kit (Beyotime, Shanghai, China) according to the manufacturer’s protocol. Images were collected by optical microscopy and analyzed by ImageJ software.
4
Cerebellar Neuroanatomy Quantification via Nissl Staining
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Nissl staining was performed using Nissl staining Kit (Beyotime) (Xie et al., 2015 (link)). Sagittal cerebellar slices (30 μm) were immersed in Nissl staining solution for 5 min, rinsed with distilled water, dehydrated in ethanol, and cleared in xylene. Images of the cerebellar cortex were captured using a light microscope. The quantification of cerebellar area, lobular thickness, and the thicknesses of granule cell and molecular layers in Nissl staining were measured as same as those in H&E staining.
5
Cerebellar Nissl Staining Protocol
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Nissl staining was performed using Nissl staining Kit (Beyotime). Sagittal cerebellar slices (30 μm) were immersed in Nissl staining solution for 5 min, rinsed with distilled water, dehydrated in ethanol, and cleared in xylene. Images of cerebellar cortex were captured using a light microscope.
6
Nissl Staining of Brain Sections
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Nissl staining was performed using Nissl staining Kit (Beyotime, C0117). Coronal slices (30 μM thickness) were immersed in Nissl staining solution for 10 min at 37 °C, rinsed with ddH2O, dehydrated in ethanol, and cleared in xylene. Images of cortex and hippocampus were captured using an Olympus VS120 microscope. Hippocampus CA1 and CA3 thickness was calculated by Image J software.
7
Histological Analysis of Brain Sections
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The paraffin embedded 4-mm thick brain sections from different group were used for H&E staining and nissl staining. The hematoxylin, eosin and the nissl staining kit were supplied by Beyotime (Beijing, China). The sections were deparaffinized in xylene, treated with a graded series of alcohol [100%, 95%, and 85% and 75%, ethanol/double-distilled H2O (v/v)], and rehydrated in PBS (Supplied by Zsbio, Beijing, Ching). The HE and nissl staining were performed following the instructions supplied by the manufacturer. Olympus B × 600 microscope and Spot Fiex camera were performed to evaluate all specimens.
8
Histological Assessment of Neuronal Damage
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HE staining kit and Nissl staining kit (Beyotime, Jiangsu, China) were applied to stain the slices according to the instructions. The extent of tissue damage was evaluated by observing the morphometric changes using an Olympus light microscope and a CCD camera (Leica DMI4000B, Germany). Furthermore, the number of survived neurons and the lesion size were quantitatively analyzed. All the procedures were performed in a blinded fashion.
9
Nissl Staining Protocol for Microscopy
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According to the manufacturer’s instructions, the Nissl staining kit (Beyotime, Shanghai, China) was used for staining (Ji et al., 2022 (link)). In brief, the sections were placed in toluidine blue at 60°C for 40 min. Then the sections were washed by distilled water. After being placed in 95% ethanol for rapid differentiation, the sections were transferred to anhydrous ethanol for rapid dehydration. Finally, the results were observed under a light microscope (×400 magnification).
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