Anti haptoglobin

Manufactured by Calibre Scientific

Anti-Haptoglobin is a laboratory product used for the detection and measurement of haptoglobin, a protein in the blood. It serves as a tool for diagnostic and research purposes.

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Lab products found in correlation

Alexa fluor 555 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Anti mouse igg hrp conjugate, by Cell Signaling Technology (2 mentions) Anti adam10, by Abcam (2 mentions) Anti rabbit igg hrp conjugate, by Cell Signaling Technology (2 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (2 mentions) Anti cd63, by BD (2 mentions) Anti cd81, by BD (2 mentions) Anti tri methyl histone h3 lys27, by Cell Signaling Technology (1 mentions)

2 protocols using anti haptoglobin

Western Blot Antibody Protocol

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Primary antibodies were used at 1–2 μg ml⁻¹ for immunoblotting: anti-ADAM10 (mouse monoclonal, Abcam ab73402), anti-CD63 (mouse monoclonal, BD Biosciences 556,019), anti-CD81 (mouse monoclonal, BD Biosciences 555,675), anti-Haptoglobin (rabbit polyclonal, Biozol, GTX 112,962–25). The following secondary antibodies were used: Alexa Fluor 488 goat anti-mouse and Alexa Fluor 555 goat anti-rabbit IgG (both from Life Technologies) and anti-mouse IgG-HRP conjugate and anti-rabbit IgG-HRP conjugate (both from Cell Signaling).

Lee J.H., Eberhardt M., Blume K., Vera J, & Baur A.S. (2020). Evidence for liver and peripheral immune cells secreting tumor-suppressive extracellular vesicles in melanoma patients. EBioMedicine, 62, 103119.

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Immunodetection of Extracellular Vesicle Markers

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Primary antibodies were used at 1–2 μg·ml⁻¹ for immunoblotting, 2 μg·ml⁻¹ for immunofluorescence, and 1–10 μg·ml⁻¹ for MELC. The following antibodies were used for immunostaining, flow cytometry, or immunoblotting: anti-ADAM10 (mouse monoclonal, ab73402; Abcam), anti-CD63 (mouse monoclonal, 556019; BD Biosciences), anti-CD81 (mouse monoclonal, 555675; BD Biosciences), anti-haptoglobin (rabbit polyclonal, GTX 112962-25; Biozol), and anti-trimethyl histone H3 Lys27 (rabbit monoclonal, #9733; Cell Signalling). The following secondary antibodies were used: Alexa Fluor 488 goat anti-mouse and Alexa Fluor 555 goat anti-rabbit IgG (both from Life Technologies) and anti-mouse IgG-HRP conjugate and anti-rabbit IgG-HRP conjugate (both from Cell Signalling).

Lee J.H., Dindorf J., Eberhardt M., Lai X., Ostalecki C., Koliha N., Gross S., Blume K., Bruns H., Wild S., Schuler G., Vera J, & Baur A.S. (2019). Innate extracellular vesicles from melanoma patients suppress β-catenin in tumor cells by miRNA-34a. Life Science Alliance, 2(2), e201800205.

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