Hdac1 sc 7872

Manufactured by Santa Cruz Biotechnology

Sourced in United States

HDAC1 (sc-7872) is a mouse monoclonal antibody that recognizes the HDAC1 protein. HDAC1 is a histone deacetylase enzyme that plays a role in the regulation of gene expression.

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Lab products found in correlation

Close spelling variants of this product

Anti-HDAC1 (sc-7872; HDAC1 (H-51): sc-7872 HDAC1

4 protocols using hdac1 sc 7872

Antibody Sourcing for Cellular Analysis

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Antibodies to SHP (sc-30169), GFP (sc-8334), actin (sc-1616), and HDAC1 (sc-7872), were purchased from Santa Cruz Biotechnology; to LSD1 (ab17721), Histone H3K4-me3 (ab8580), and F4/80 (ab6640) from Abcam; and to H3K9/K14-Ac (06-599), H3K9-me2 (07-030), and Prox1 (07-537) from Millipore. Prox1 siRNA was purchased from Dharmacon.

Kim Y.C., Fang S., Byun S., Seok S., Kemper B, & Kemper J.K. (2015). FXR-induced lysine-specific histone demethylase, LSD1, reduces hepatic bile acid levels and protects the liver against bile acid toxicity. Hepatology (Baltimore, Md.), 62(1), 220-231.

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Western Blot Analysis of Spliceosomal Proteins

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Samples were prepared in 5× Laemmli buffer (62.5 mM Tris, 25% glycerol, 6.25% SDS, 0.1% bromophenol blue, 5% beta-mercaptoethanol) and heated at 95°C for 2 min prior to separation by 10% SDS-PAGE. Gels were transferred to PVDF membrane (Bio-Rad Mini Trans-Blot Cell) and blocked in 1% nonfat milk in 1× Tris-buffered saline with Tween 20 (TBST) for 1 h at room temperature while rocking. The following antibodies were added directly to the blocking buffer at the indicated concentrations and incubated at 4°C overnight while rocking. From Proteintech: DHX15 (12265-1-AP, 1:1000), SNRPB2 (13512-1-AP, 1:2500), and U2AF1 (60289-1-Ig, 1:1000). From Santa Cruz Biotech: SF3A2 (sc-390444, 1:1000) and HDAC1 (sc-7872, 1:5000). Blots were washed three times in 1× TBST, and corresponding LICOR secondaries (1:15000) were added in blocking buffer and rocked at room temperature for 1 h. The blots were again washed three times and then imaged on a LICOR Imaging System. Images were quantified and processed utilizing LICOR Image Studio Lite.

Maul-Newby H.M., Amorello A.N., Sharma T., Kim J.H., Modena M.S., Prichard B.E, & Jurica M.S. (2022). A model for DHX15 mediated disassembly of A-complex spliceosomes. RNA, 28(4), 583-595.

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Western Blot Analysis of Signaling Proteins

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Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS) supplemented with 1 mM PMSF, 1 μg/ml aprotinin, 1 μg/ml leupeptin. Protein concentration was measured by BCA Protein Assay (Pierce). Total protein (10 μg) was resolved on 10% SDS-PAGE, transferred to PVDF membranes (Immobilon-P, Millipore), and probed with primary antibodies against MTA2 (sc-55566), HDAC1 (sc-7872), SIRT1 (sc-15404) and p53 (sc-126) (Santa Cruz); YY1 (#2185), p21 (#2946), PUMA (#4976), CDK4 (#2906), CDK6 (#3136) and FAS (#8023) (Cell Signaling); MDM4 (mAb 8C6) and MDM2 (mAb 2A10) (EMD Bioscience); or anti-HA (clone 3F10, Roche), followed by incubation with horseradish peroxidase-(HRP) conjugated sheep anti-mouse or anti-rabbit Ig (Amersham). Signals were developed using SuperSignal West Femto (Pierce). Membranes were stripped and reprobed using α-tubulin mouse mAb (B-5-1-2, Sigma) or β-actin (JLA20, Millipore). Immunoblots were quantified by densitometry using VersaDoc MP 4000 (Bio-Rad).

Navarro F, & Lieberman J. (2015). miR-34 and p53: New Insights into a Complex Functional Relationship. PLoS ONE, 10(7), e0132767.

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Doxorubicin-Induced Mitochondrial Dysfunction

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Doxorubicin, neferine, DCF-DA, FURA 2/AM, JC-1, DAPI, rotenone, lysis buffer (CelLytic™), U0126 ERK1/2 and SB203580 p38 inhibitor were purchased from Sigma (Bangalore, India). Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS) and antibiotic solutions were obtained from HiMedia Laboratories (Mumbai, India). DiOC6 (3,3′-dihexyloxacarbocyanine iodide) and CCCP [carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] were obtained from Calbiochem, (San Diego, CA, USA). PVDF membranes were obtained from Whatman (Clifton, NJ, USA). Protein marker (PageRuler™) and all other chemical used for SDS-PAGE were obtained from Thermo Scientific (Bangalore, India). Primary monoclonal antibodies for p22phox (sc-20781), p47phox (sc-14015), gp91phox/Nox2 (sc-5827), p-ERK1/2 (sc-7383), p-p38 (sc-7973), NfκB (sc-109), COX2 (sc-1745), TNF-α (sc-1350), Bcl-2 (sc-7382), Bax (sc-526), p-Bad (sc-101641), cytochrome C (sc-13560), caspase-9 (sc-8355), β-actin (sc-47778), α-tubulin (sc-5286), cyclin D1 (sc-246), GAPDH (sc-25778), HDAC1 (sc-7872) and HRP conjugated secondary antibodies raised against mouse, rabbit and goat were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against caspase-3 (#9665 s) was purchased from Cell Signalling Technology (Danver, MA 01923, USA).

Priya L.B., Baskaran R., Huang C.Y, & Padma V.V. (2017). Neferine ameliorates cardiomyoblast apoptosis induced by doxorubicin: possible role in modulating NADPH oxidase/ROS-mediated NFκB redox signaling cascade. Scientific Reports, 7, 12283.

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