Avance 3 300 spectrometer

NMR spectroscopic measurements were performed on a Bruker Avance III-300 spectrometer (operating at 300 MHz for ¹H NMR and 75 MHz for ¹³C) and on a Bruker Avance III-500 spectrometer (operating at 500 MHz for ¹H NMR and 126 MHz for ¹³C) from Bruker Biospin GmbH (Rheinstetten, Germany) at 20 °C. All chemical shifts (δ) are reported in ppm relative to solvent residual signals of the deuterated solvent.

To study the bond structure of SCMP, the as-prepared polymer was characterized via Fourier transform infrared (FTIR) spectroscopy and ¹³C nuclear magnetic resonance (NMR) spectroscopy (Bruker AVANCE III 300 Spectrometer). Powder X-ray diffraction (XRD, Bruker) patterns were obtained using Cu Kα radiation (λ = 1.5406 Å). The morphologies and structures of the samples were observed via scanning electron microscopy (SEM, S-4800, Hitachi) and transmission electron microscopy (TEM, JEM-2010, JEOL). A multifunctional X-ray photoelectron spectrometer (XPS, ESCALAB 250XI, Thermo Scientific) was employed to analyze the chemical states and compositions of the samples. Raman spectra were obtained using a Raman spectrometer (RM-1000, Renishaw) with a laser having a wavelength of 632.8 nm. The carbon conversion rate of the SCMP was characterized via thermogravimetric analysis (TGA, TGA/DSC 1/1600HT, Metter), under heating from room temperature to 1000 °C with a heating rate of 10 °C min⁻¹ in a N₂ atmosphere. The N₂ adsorption–desorption isotherms were obtained at 77 K using an Autosorb iQ2 system (Quantachrome Instruments), and the specific surface area and pore-size distribution were calculated from the adsorption data using the Brunauer–Emmett–Teller (BET) model.

NMR spectra were recorded either on a BRUKER AVANCE III 300 spectrometer (¹H, 300 MHz and ¹³C{¹H}, 75 MHz) or on a BRUKER AVANCE III 400 (²⁹Si{¹H}, 79.6 MHz) spectrometer. Chemical shifts are calibrated to trimethylsilane (TMS) based on the relative chemical shift of the residual non-deuterated solvent as an internal standard given in ppm relative to TMS and coupling constants J in Hz.

Cobalt(II)
acetate tetrahydrate and NaHCO₃ were used
as received from Merck (Germany). Commercial-grade methanol, distilled
over CaCl₂, was used for synthesis of the complexes. IR
(attenuated total reflectance, ATR) spectra were recorded on a Nicolet
iS10 spectrometer at ambient temperature. Elemental analyses were
run on a Vario EL instrument from Elementar Analysensysteme. EI-MS
spectra were acquired with a Thermo-Finnigan TSQ 700 spectrometer.
Isotopic distribution patterns for ^58/60Co [in Co-(R or S)-L1 and Co-(R or S)-L2] or combined ^58/60Co + ^79/81Br [Co-(R or S)-L3] containing
ions are detected in the mass spectra. DSC was run on a Shimadzu DSC-60A
heat-flux instrument (working in endodown mode) in the range 30–240
°C (just up to decomposition temperature) with a rate of 10 K
min^–1 under a nitrogen atmosphere. NMR spectra were
measured on a Bruker Avance III-300 spectrometer.

Samples of hydrated larvae P. vanderplanki used for the NMR experiment were in full active life. ¹H-NMR spectra were collected on Bruker Avance III 300 spectrometer (Bruker Biospin), operating at the resonance frequency 300 MHz (at B₀ = 7 T), with the transmitter power used equal to 400 W. The pulse length was π/2 = 2.2 μs (corresponding to wide excitation bandwidth of 300 kHz) and repetition time was 3 s. Under this experimental conditions it was possible to examine both anhydrobiotic and live larvae. The data obtained were analyzed using commercially available software Origin 9.0, OriginLab Corporation, USA.