Rad001

Bosutinib, masitinib and midostaurin (PKC412) were purchased from LC Laboratories (Woburn, MA, USA), ponatinib from Selleck Chemicals (Houston, TX, USA), piceatannol and pimozide from Sigma Aldrich (San Louis, MO, USA), and dasatinib, sorafenib, sunitinib, tozasertib, vorinostat, everolimus (RAD001), erlotinib, gefitinib, and lapatinib from ChemieTek (Indianapolis, IN, USA). Imatinib, nilotinib, and BEZ235 were kindly provided by Dr.E.Buchdunger and Dr.P.W.Manley (Novartis, Basel, Switzerland). Stock solutions of drugs were prepared by dissolving in DMSO (Merck, Darmstadt, Germany). A specification of targeted drugs is shown in Supplementary Table S1. RPMI 1640 medium and fetal calf serum (FCS) were from PAA Laboratories (Pasching, Austria), ³H-thymidine from Amersham (Buckinghamshire, United Kingdom), and the Annexin V-FITC Kit from eBiosciences (San Diego, CA, USA). Recombinant human (rh) stroma cell-derived factor-1 alpha (SDF-1α) and rh eotaxin were purchased from R&D Systems (Minneapolis, MN, USA), and rh interleukin-5 (IL-5) from BD Bioscience (San José, CA, USA). A specification of monoclonal antibodies (mAb) used in this study is shown in Supplementary Table S2.

Masitinib and midostaurin (PKC412) were purchased from LC Laboratories (Woburn, MA, USA), piceatannol and pimozide from Sigma-Aldrich (St Louis, MO, USA), RDEA119, PD0325901 and NVP-BEZ235 from Selleck (Houston, TX, USA) and RAD001 from ChemieTek (Indianapolis, IN, USA). The antibody-drug conjugate brentuximab vedotin (SGN-35) was kindly provided by Dr P. Veiby and Dr J. V. Garafalo (Millennium Takeda Oncology Company, Cambridge, MA, USA). Stock solutions of drugs were prepared by dissolving in dimethyl sulfoxide (Merck, Darmstadt, Germany). Recombinant human (rh) interleukin (IL)-2 was obtained from ImmunoTools (Friesoythe, Germany), rhIL-4 from Peprotech (Rocky Hill, NJ, USA), rhIL-5 from BD Biosciences (San Jose, CA, USA), rhIL-6 from Novartis (Basel, Switzerland), rhIL-13, rhCD30 ligand, recombinant canine (rc) IL-4, and rc stem cell factor (SCF) from R&D Systems (Minneapolis, MN, USA) and rhSCF from Strathmann Biotech (Hannover, Germany). RPMI 1640 medium and fetal calf serum (FCS) were purchased from PAA Laboratories (Pasching, Austria), ³H-thymidine from Amersham (Buckinghamshire, UK) and the Annexin V-FITC Kit from eBiosciences (San Diego, CA, USA).