Igg1 rtk2071

Manufactured by BioLegend

IgG1 (RTK2071) is a monoclonal antibody that belongs to the IgG1 isotype. It is produced by BioLegend and can be used for various research applications.

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Lab products found in correlation

Histopaque, by Merck Group (1 mentions) Facs caliber, by BD (1 mentions) Nlrp3 clone d4d8t, by Cell Signaling Technology (1 mentions) Anti il 1β, by Merck Group (1 mentions) Anti nlrp3, by Abcam (1 mentions) Brdu flow kit, by BD (1 mentions) Cucurbitacin 1, by Merck Group (1 mentions) Cytotox 96 non radioactive cytotoxicity assay kit, by Promega (1 mentions) Ova257 264 siinfekl, by AnaSpec (1 mentions) Polybrene, by Merck Group (1 mentions)

3 protocols using igg1 rtk2071

Profiling T Cell Infiltration in Mammary Tumors

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For analysis of T cell infiltration and activation, MMTV-PyMT mammary tumor tissues were digested with collagenase D (1mg/mL) and DNase I (100μg/mL), and cell suspensions were filtered through a 70 μm cell strainer as previously described [75 (link)-76 (link)]. The viable mononuclear cells were isolated using the Histopaque (Sigma-Aldrich) gradient, and analyzed using a FACScaliber (BD Biosciences). To determinate the activation of CD8⁺ T cells, single cell suspension prepared from PyMT mammary tumors were stimulated with PMA plus ionomycin in the presence of Golgi-stop for 6 hours, followed by intracellular staining for IFN-γ or granzyme B-producing CD8⁺ cells. Fluorochrome-conjugated mouse mAbs, including FITC-CD8 (53-6.7), APC-CD4 (GK1.5) and PE-IFN-γ (XMG1.2), as well as CD16/CD32 (2.4G2), isotype control rat IgG2b (RTK4530), and IgG1 (RTK2071) were purchased from BioLegend (San Diego, CA).

Menezes M.E., Shen X.N., Das S.K., Emdad L., Guo C., Yuan F., Li Y.J., Archer M.C., Zacksenhaus E., Windle J.J., Subler M.A., Ben-David Y., Sarkar D., Wang X.Y, & Fisher P.B. (2015). MDA-7/IL-24 functions as a tumor suppressor gene in vivo in transgenic mouse models of breast cancer. Oncotarget, 6(35), 36928-36942.

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Flow Cytometry Analysis of Immune Cells

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Fluorochrome-conjugated mouse monoclonal antibodies (mAbs) for flow cytometry analysis of immune cells, including FITC-CD4 (GK1.5), Percp/Cy5.5–IL-17A (TC11-18H10.1), CD16/CD32 (2.4G2), isotype control rat IgG2b (RTK4530), and IgG1 (RTK2071) were purchased from BioLegend (San Diego, CA). Mouse TNF-α, IL-6, and IL-1β enzyme-linked immunosorbent assay (ELISA) kits were purchased from Biolegend. Monoclonal antibodies for ASC (clone 2EI-7, 04-147, EMD Millipore, Billerica, MA), and NLRP3 (clone D4D8T, Cell Signaling Biotechnology) were purchased from commercial resources as indicated. Anti-NLRP3 (Abcam, U.S.A.), anti-IL-1β (EMD Millipore, CA, U.S.A.), and anticaspase-1 (p20 and p10) (Santa Cruz Biotechnology, Santa Cruz, U.S.A.) for Western blotting analysis were purchased from commercial resources as indicated and used per manufacturer’s instruction.

Guo C., Fulp J.W., Jiang Y., Li X., Chojnacki J.E., Wu J., Wang X.Y, & Zhang S. (2017). Development and Characterization of a Hydroxyl-Sulfonamide Analogue, 5-Chloro-N-[2-(4-hydroxysulfamoyl-phenyl)-ethyl]-2-methoxy-benzamide, as a Novel NLRP3 Inflammasome Inhibitor for Potential Treatment of Multiple Sclerosis. ACS chemical neuroscience, 8(10), 2194-2201.

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Cytokine-Mediated CD8+ T Cell Activation

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OVA_257–264 (SIINFEKL) and gp100_25–33 (KVPRNQDWL) peptides were purchased from AnaSpec Inc (Fremont, CA). Recombinant human IL-2, IL-7 and IL-15 cytokines were kindly provided by the National Cancer Institute (NCI, MD). For fluorescence-activated cell sorting (FACS) analysis, mouse monoclonal antibodies to CD4 (GK1.5), CD8a (53–6.7), CD3 (HL3), IFN-γ (XMG1.2), CD25 (PC61), CD90.1 (OX-7), isotype control rat IgG2b (RTK4530), and IgG1 (RTK2071) were purchased from BioLegend (San Diego, CA); FoxP3 antibody (FJK-16s) was purchased from eBioscience (San Diego, CA). For Western blotting analysis, anti-human MDA-7/IL-24 antibody was purchased from GenHunter (Nashville, TN, USA); anti-mouse MDA-7/IL-24 antibodies were purchased from R&D Systems (Minneapolis, MN); phosphor-STAT1 (Y701), phosphor-STAT3 (Y705), total STAT1 and total STAT3 antibodies were purchased from Cell Signaling Technology (Davers, MA). Cucurbitacin I was purchased from Sigma Aldrich (St. Louis, MO). BrdU Flow Kit was purchased from BD Biosciences (San Jose, CA). Polybrene was purchased from Sigma Aldrich (St. Louis, MO). CytoTox 96^® Non-Radioactive Cytotoxicity Assay kits were purchased from Promega (Madison, WI). Cell Counting Kits 8 (CCK8) were purchased from Abcam (Cambridge, MA).

Liu Z., Guo C., Das S.K., Yu X., Pradhan A.K., Li X., Ning Y., Chen S., Liu W., Windle J.J., Bear H.D., Manjili M.H., Fisher P.B, & Wang X.Y. (2021). Engineering T cells to express tumoricidal MDA-7/IL-24 enhances cancer immunotherapy. Cancer research, 81(9), 2429-2441.

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