Paraffin wax was purchased from Leica Microsystems, Inc. (Wetzlar, Germany).
Blank paraffin blocks prepared for the recipient blocks were made according to
routine procedures. Briefly, ordinary stainless-steel embedding molds and
plastic cassettes were placed in melted paraffin and warmed to 62°C before
filling to avoid the formation of bubbles in the paraffin blocks. The mold was
then filled with melted paraffin and covered with a plastic cassette. After
pouring, the paraffin blocks were cooled to room temperature and removed from
the steel molds. These blank paraffin blocks were examined, and those without
air bubbles and cracks were selected for to prepare the recipient block. The
paraffin block with a plastic cassette was placed at the bottom of the plastic
mold to fix the paraffin block and avoid its displacement during drilling. Holes
were created in the recipient block using a mini-drill. The recipient block was
removed after drilling was completed.
Blank paraffin blocks prepared for the recipient blocks were made according to
routine procedures. Briefly, ordinary stainless-steel embedding molds and
plastic cassettes were placed in melted paraffin and warmed to 62°C before
filling to avoid the formation of bubbles in the paraffin blocks. The mold was
then filled with melted paraffin and covered with a plastic cassette. After
pouring, the paraffin blocks were cooled to room temperature and removed from
the steel molds. These blank paraffin blocks were examined, and those without
air bubbles and cracks were selected for to prepare the recipient block. The
paraffin block with a plastic cassette was placed at the bottom of the plastic
mold to fix the paraffin block and avoid its displacement during drilling. Holes
were created in the recipient block using a mini-drill. The recipient block was
removed after drilling was completed.