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Anti human glycoprotein 6 purified antibody

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Anti-human Glycoprotein VI purified antibody is a laboratory reagent used for the detection and analysis of the human Glycoprotein VI protein. Glycoprotein VI is a transmembrane receptor found on the surface of platelets and plays a role in platelet activation and aggregation. This antibody can be used in various immunological techniques, such as Western blotting, ELISA, and flow cytometry, to study the expression and function of Glycoprotein VI in biological samples.

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3 protocols using anti human glycoprotein 6 purified antibody

1

Salidroside Modulates Platelet Activation

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Salidroside was purchased from MedChemExpress (Monmouth Junction, NJ, USA) with a purity ≥ 98%. Collagen-related peptide (CRP) was prepared as previously described [56 (link)]. Thrombin (≥ 10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, USA). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, USA) and BECTON DICKINSON (San Jose, CA, USA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). SB216763 (GSK-3β inhibitor) was purchased from ApexBio Technology.
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2

Platelet Aggregation Inhibition by Platycodin D

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Platycodin D (PD) was purchased from Fluka (Steinheim, Germany) with a purity ≥ 99%. Collagen, adenosine diphosphate (ADP), arachidonic acid and epinephrine were from Helena laboratories (Beaumont, Texas, USA). Thrombin was purchased from Sigma-Aldrich (St. Louis, MO, USA). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, USA) and Becton–Dickinson (San Jose, CA, USA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA).
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3

Platelet Activation Assay Using Flow Cytometry

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ATRA was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in 0.5% v/v (final concentration) DMSO. Collagen and thrombin (≥ 10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, USA). Collagen-related peptide (CRP) was prepared as previously described (18) . FITC-conjugated mouse anti-human CD41a was from BD Biosciences (San Jose, CA, USA). PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). -actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Tirofiban was purchased from Grand Pharma (China) CO. LTD.
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