Total RNA was isolated from cells with TRIzol followed by RNAeasy mini Kit After DNase I treatment (TURBO DNA free, Ambion), cDNA was synthesized from 1 μg purified RNA by the SuperScript II First-Strand cDNA synthesis system. Quantitative PCR was performed in 20 μL reactions using SsoAdvanced SYBR Green Supermix in BioRad CFX 96 Touch Real Time PCR System. For primers, see Key resources table . PCR reactions were performed in triplicate. Experiments included template-free (water) and reverse transcriptaseminus controls to prevent contamination. Relative mRNA quantities in experimental samples were determined by CFX Maestro 1.0 software, normalized to housekeeping gene as indicated, and expressed in arbitrary units as fold-difference from control.
Rnaeasy mini kit after dnase 1 treatment
Manufactured by Thermo Fisher Scientific
The RNAeasy mini Kit After DNase I treatment is a laboratory equipment product designed to purify RNA from various sample types. It utilizes a silica-membrane-based technology to efficiently capture and elute RNA, with the inclusion of a DNase I treatment step to remove any contaminating DNA. The core function of this kit is to provide a reliable and effective method for isolating high-quality RNA for downstream applications.
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2 protocols using rnaeasy mini kit after dnase 1 treatment
1
Quantitative RNA Expression Analysis
2
Quantitative RNA Expression Analysis
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Total RNA was isolated from cells with TRIzol followed by RNAeasy mini Kit After DNase I treatment (TURBO DNA free, Ambion), cDNA was synthesized from 1 μg purified RNA by the SuperScript II First-Strand cDNA synthesis system. Quantitative PCR was performed in 20 μL reactions using SsoAdvanced SYBR Green Supermix in BioRad CFX 96 Touch Real Time PCR System. For primers, see Key resources table . PCR reactions were performed in triplicate. Experiments included template-free (water) and reverse transcriptaseminus controls to prevent contamination. Relative mRNA quantities in experimental samples were determined by CFX Maestro 1.0 software, normalized to housekeeping gene as indicated, and expressed in arbitrary units as fold-difference from control.
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