Western blots were conducted as previously described (Markovics 2005 (link)) with the following antibodies: Total STAT1 (Cell Signal #9172), Phospho-STAT1 Ser727 (Cell Signal #9177), and Total STAT2 (Cell Signal #4597) and were prepared in accordance with the manufacturer’s instructions. SV40 T Antigen mouse monoclonal antibodies have been described previously: PAb416, PAb419 (Harlow 1981 (link)), and PAb901 (Fu 1996 (link)). Additional mouse monoclonal antibodies against JCV T antigens (PAb962 and AB2003) were previously described (Bollag 2000 (link), Munoz-Marmol 2004 (link)). GAPDH mouse monoclonal was used as the loading control (US Biologicals #G8140-11). Peroxidase-conjugated goat anti-mouse (A2554), and goat anti-rabbit (A0545) from Sigma-Aldrich were used as secondary antibodies. The Luminata Western HRP substrate (Millipore #WBLUF0100) was used in accordance with manufacturer’s instructions. A mixture of monoclonal antibodies 416/419/962/2003 (1:1000/1:500/1:1000/1:1000) were used for the detection of T antigen signal in Figures 2C , 3A , 4B , and 7B . Additionally, SV40-C257 detection used monoclonal Ab901 (1:500) Figure 5D , and in Figure 5B a mixture of 416/419 (1:500/1:250) was used.
Peroxidase conjugated goat anti mouse a2554
Manufactured by Merck Group
Peroxidase-conjugated goat anti-mouse (A2554) is a secondary antibody that targets mouse primary antibodies. It is labeled with horseradish peroxidase (HRP), an enzyme that can be used for signal detection in various immunoassays and immunochemical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Luminata western hrp substrate, by Merck Group (1 mentions)
Total stat1, by Cell Signaling Technology (1 mentions)
Phospho stat1 ser727, by Cell Signaling Technology (1 mentions)
Total stat2, by Cell Signaling Technology (1 mentions)
Sodium oxamate, by Merck Group (1 mentions)
Peptide n glycosidase f pngase f, by New England Biolabs (1 mentions)
D mannose, by Merck Group (1 mentions)
Irdye 680rd donkey anti mouse, by LI COR (1 mentions)
Anti β actin antibody, by Cell Signaling Technology (1 mentions)
2 deoxy d glucose 2 dg, by Merck Group (1 mentions)
Tunicamycin, by Merck Group (1 mentions)
2 protocols using peroxidase conjugated goat anti mouse a2554
1
Western Blot Analysis of STAT and T Antigens
2
Glycosylation Assay with Antiviral Agents
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2-Deoxy-D-glucose (2-DG), sodium oxamate, D-mannose, and tunicamycin were purchased from Sigma and used at the indicated final concentrations. Peptide N-Glycosidase F (PNGase F) (P0708S) was obtained from New England BioLabs. Anti-β-actin antibody (#3700) was purchased from Cell Signaling Technology, anti-α-tubulin antibody (ab7291) was purchased from Abcam, anti-GP2 antibody (83.6) was a kind gift from Prof. Stefan Kunz (Institute of Microbiology of the University of Lausanne, Lausanne, Switzerland), anti-NP (M87) and anti-Z (MJ-3) are in-house made monoclonal antibodies [14 (link)]. Peroxidase-conjugated goat anti-mouse (A2554) was purchased from Sigma, and IRDye® 680RD donkey anti-mouse (926-68072) was from Li-Cor.
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