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Mouse polyclonal anti actb

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Mouse polyclonal anti-ACTB is a laboratory reagent used for the detection and measurement of the ACTB (actin beta) protein. ACTB is a housekeeping gene that encodes a cytoskeletal protein involved in various cellular processes. This polyclonal antibody is generated in mice and can be used in various immunoassay applications to identify and quantify ACTB expression.

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Lab products found in correlation

Nitrocellulose, by GE Healthcare (2 mentions) Lithium dodecyl sulfate (lds), by Merck Group (2 mentions) Nupage bis tris mini gel, by Thermo Fisher Scientific (2 mentions) Mouse monoclonal anti fli1, by Abcam (2 mentions) Enhanced chemi luminescence (ecl), by Cytiva (1 mentions) Trizma hydrochloride buffer solution, by Merck Group (1 mentions)

Spelling variants of this product

Anti-ACTB (10 citations) Mouse anti-ACTB (2 citations)

2 protocols using mouse polyclonal anti actb

1

Western Blot Analysis of Cell Signaling

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TC32 cells (1.5 × 106) were incubated with drug, collected, washed with PBS, lysed, and boiled in 4% LDS buffer (0.125 M Trizma hydrochloride buffer solution, pH 7.5, and 4% lithium dodecyl sulfate (Sigma-Aldrich). Protein concentrations were determined after diluting the detergent using the bicinchoninic acid (BCA) assay kit (Pierce Protein Biology Products, Carlsbad, CA). Thirty micrograms of protein was resolved on a 4–12% NuPAGE Bis-Tris Mini gels (Invitrogen, Carlsbad, CA) in 1 × 4-morpholinepropanesulfonic acid (MOPS) sodium dodecyl sulfate (SDS) buffer (Invitrogen), and transferred to nitrocellulose (GE Healthcare Life Sciences, Pittsburgh, PA). The membrane was probed with the following antibodies: mouse monoclonal anti-FLI1 (1:1,000, Abcam, Cambridge, MA), mouse polyclonal anti-ACTB (1:1,000, Cell Signaling, Danvers, MA), rabbit polyclonal anti-ENT1 (1:500, Abcam), rabbit polyclonal anti-ENT2 (1:1000, Abcam), and rabbit polyclonal anti-TK1 (1:000, Santa Cruz Biotechnology, Dallas, TX). The protein was visualized by using horseradish peroxidase (HRP)-conjugated secondary antibody and ECL (Amersham, Buckinghamshire, UK).
Osgood C.L., Tantawy M.N., Maloney N., Madaj Z.B., Peck A., Boguslawski E., Jess J., Buck J., Winn M.E., Manning H.C, & Grohar P.J. (2016). 18F-FLT Positron Emission Tomography (PET) is a Pharmacodynamic Marker for EWS-FLI1 Activity and Ewing Sarcoma. Scientific Reports, 6, 33926.
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2

Western Blot Analysis of Protein Expression

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TC32 and TC71 cells (1.5 × 106) were incubated with drug, collected, washed with PBS, lysed and boiled in 4% LDS buffer (0.125 M Trizma hydrochloride buffer solution, pH 7.5, and 4% lithium dodecyl sulfate (Sigma-Aldrich, St. Louis, MO). Protein concentrations were determined after diluting the detergent using the bicinchoninic acid (BCA) assay kit (Pierce Protein Biology Products, Carlsbad, CA). Thirty micrograms of protein was resolved on a 4–12% NuPAGE Bis-Tris Mini gels (Invitrogen, Carlsbad, CA) in 1x 4-morpholinepropanesulfonic acid (MOPS) sodium dodecyl sulfate (SDS) buffer (Invitrogen), transferred to nitrocellulose (GE Healthcare Life Sciences, Pittsburgh, PA) and probed with the following antibodies: rabbit monoclonal anti-EZH2 (1:1,000, Cell Signaling, Danvers, MA), mouse monoclonal anti-FLI1 (1:1,000, Abcam, Cambridge, MA), mouse polyclonal anti-ACTB (1:1,000, Cell Signaling), rabbit polyclonal anti-NR0B1 (1:500, Abcam), mouse monoclonal anti-phospho-histone H2A.X (Ser139) (1:1,0000, Millipore, Billerica, MA), and rabbit monoclonal anti-ID2 (1:1,000, Cell Signaling). The protein was visualized by using horseradish peroxidase (HRP)-conjugated secondary antibody and ECL (Amersham, Buckinghamshire, UK).
Osgood C.L., Maloney N., Kidd C.G., Kitchen-Goosen S., Segars L., Gebregiorgis M., Woldemichael G.M., He M., Sankar S., Lessnick S., Kang M., Smith M., Turner L., Madaj Z.B., Winn M.E., Núñez L.E., González-Sabín J., Helman L.J., Morís F, & Grohar P.J. (2016). Identification of mithramycin analogs with improved targeting of the EWS-FLI1 transcription factor. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(16), 4105-4118.
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