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2 protocols using alfuzosin

1

Breast Cancer Cell Line Cultivation Protocol

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Gefitinib, lapatinib, terazosin, alfuzosin, prazosin, irinotecan, and quinacrine were purchased from Sigma Chemical (St. Louis, MO, USA). The breast cancer cell lines used in this study, MCF-7 and MDA-MB-231, were obtained from the American Type Tissue Culture Collection (ATCC), Rockville, MD, USA. MCF-7 and MDA-MB-231 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) high glucose with phenol red, and the culture medium was supplemented with 10% fetal bovine serum (FBS) (BioWest, Riverside, MO, USA) and 1% penicillin/streptomycin as an antibiotic. The cells were incubated in culture flasks (75 cm3) at 37 °C in a humidified atmosphere of 5% CO2 and 95% air, all of which were carried out under sterile conditions using a laminar flow hood.
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2

Compound Screening for Ion Channel Modulation

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Sertindole (CAS # 106516-24-9) and Moxifloxacin (151096-09-2) were purchased from ChemPacific (MD, USA), L768673 (Selnick et al., 1997 (link)) was purchased from Albany Molecular Research Inc. (N Y, USA), Cisapride (81098-60-4) was from Tocris Bioscience (MO, USA), Ranolazine, Alfuzosin, Mexiletine, Flecainide, Terfenadine, Lamotrigine, DL-sotalol, and Terodiline were from Sigma-Aldrich (MO, USA), Dofetilide was synthesized at American Custom Chemicals Corporation (San Diego, CA), Tolterodine (214601-13-5) was from Toronto Research Chemicals (Toronto, Canada), and AMG 1 was synthesized at Amgen Medicinal Chemistry (Thousand Oaks, CA). All compounds were dissolved in DMSO to make stock solutions.
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