GC-MS analysis of MH was performed using GC-MS (Shimadzu QP2010 Ultra system, Kyoto, Japan) equipped with a capillary column Rxi-5ms (30 m length × 0.25 mm ID × 0.25 μm film thickness). The programmed temperatures were as follows: injector temperature was 250°C, ion-source temperature was 200°C, oven temperature was initially 50°C which was then increased at 3°C/min to 300°C and held for 10 min. Helium was used as the carrier gas at a pressure of 37.1 kPa. The mass spectra of separated components were identified based on data from WILEY and National Institute of Standards and Technology (NIST) libraries.
Qp2010 ultra system
Manufactured by Shimadzu
Sourced in Japan
The QP2010 Ultra system is a high-performance gas chromatograph-mass spectrometer (GC-MS) designed for analytical applications. It offers precise and accurate analysis of a wide range of organic compounds. The system combines a gas chromatograph and a quadrupole mass spectrometer to provide comprehensive separation and identification of complex sample mixtures.
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Lab products found in correlation
6 protocols using qp2010 ultra system
1
GC-MS Analysis of Methamphetamine
2
Quantifying Cecal Short-Chain Fatty Acids
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The contents of SCFAS in the ceca were analyzed using gas chromatography–mass spectrometry (GC-MS) as previously described [16 (link)], including acetic, propionic, and butyric acid. The cecal contents were freeze-dried and approximately 50 mg of the samples were suspended with saturated NaCl for 30 min. SCFAs were then extracted by diethyl ether and then quantified by GC-MS (QP2010 Ultra system, Shimadzu Corporation, Kyoto, Japan).
3
Characterization of Mengkudu Essential Oil
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The MEO was diluted with hexane to 1% (v/v) and analyzed by means of a QP-2010 Ultra system (Shimadzu, Tokyo, Japan). The gas chromatograph-mass spectrometry (GC-MS) analysis was performed as previously described [19 (link)]. An HP-5MS capillary column (30 m × 250 µm, film thickness 0.25 µm) was used for the separations. The temperature program was maintained at 50 °C for 1 min, increased to 250 °C at a rate of 5 °C /min, and maintained for a further 5 min. The column pressure was 50 kPa, and the carrier gas (helium, 1.2 mL/min) was at a split ratio of 1:50. The MS was operated in electron impact mode. The ionization energy was 70 eV. The ion source temperature was 230 °C, scanning mass range of 20–500 amu.
The identification of MEO chemical compounds were done using GC-MS software (GC-MS Postrun Analysis) and computer matched within the NIST14 library. The relative percentage of components in the MEO calculated by peak area normalization.
The identification of MEO chemical compounds were done using GC-MS software (GC-MS Postrun Analysis) and computer matched within the NIST14 library. The relative percentage of components in the MEO calculated by peak area normalization.
4
Quantifying Fecal Short-Chain Fatty Acids
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Rat feces were collected 1 d before the end of the study period. We analyzed the feces for short-chain fatty acids (SCFA), including acetate, propionate, and butyric acid using GC-MS (QP-2010 Ultra system, Shimadzu Corporation, Kyoto, Japan). Fecal samples (200 mg) were collected directly into sterile tubes from live rats, homogenized in 1 mL of sterile water, and then oscillated for 10 min. The suspension was centrifuged at 18,800 × g for 10 min, and the supernatant was centrifuged again at 18,800× g for 15 min. The supernatant was subjected to 0.45 μm membrane filtration. The filtrate (1 mL) and formic acid (0.1 mL) were taken into a gas-phase flask for SCFA analysis (Qu et al., 2018) (link).
5
GC-MS Analysis of Volatile Compounds
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GC-MS was performed as previously
described[13 (link)] using a QP2010Ultra system (Shimadzu
Corporation, Kyoto, Japan) with a DF:0.25-mm
InertCap 5MS/NP column (30 m × 0.25 mm internal
diameter; GL-Science, Tokyo, Japan). The inlet
temperature was 230°C and the column flow rate was
1.12 mL/min. Helium was used as the carrier gas.
The column temperature was maintained at 80°C for
2 min, increased to 320°C at a rate of 15°C/min,
and held for 6 min. The transfer line and source
temperatures were 250°C and 200°C, respectively.
Electron ionization was performed at 70 V. Twenty
scans per second were recorded over a mass range
of 85–500 m/z. In addition, a standard alkane
mixture (C9-C40) was injected through the column
before sampling. The retention time data for each
peak in the mixture were used as a reference for
tentative identification.
described[13 (link)] using a QP2010Ultra system (Shimadzu
Corporation, Kyoto, Japan) with a DF:0.25-mm
InertCap 5MS/NP column (30 m × 0.25 mm internal
diameter; GL-Science, Tokyo, Japan). The inlet
temperature was 230°C and the column flow rate was
1.12 mL/min. Helium was used as the carrier gas.
The column temperature was maintained at 80°C for
2 min, increased to 320°C at a rate of 15°C/min,
and held for 6 min. The transfer line and source
temperatures were 250°C and 200°C, respectively.
Electron ionization was performed at 70 V. Twenty
scans per second were recorded over a mass range
of 85–500 m/z. In addition, a standard alkane
mixture (C9-C40) was injected through the column
before sampling. The retention time data for each
peak in the mixture were used as a reference for
tentative identification.
6
Fecal SCFA Quantification by GC-MS
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The concentrations of SCFAs were determined according to previous reports using gas chromatography-mass spectrometry (QP2010 Ultra System, Shimadzu Corporation, Kyoto, Japan) with an Rtx-Wax column (30 × 0.25 × 0.25 m)29 (link). Briefly, 200 mg fecal samples were vortexed with 1 mL aqua distillate for 10 min and centrifuged at 5000 rpm at 4 °C for 10 min. Subsequently, 500 µL of the supernatant was filtrated using a 0.22 µm Millipore filter and thoroughly mixed with 50 µL of 50% sulfuric acid solution (v/v). Then 800 µL diethyl ether was used for SCFAs extraction for 20 min and centrifuged at 10,000 rpm at 4 °C. On the 7th, 14th, and 21st days, the contents of acetic, propionic, butyric, and total organic acids were detected in animal feces. The fecal concentration of SCFAs was calculated according to the standard curve.
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