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Cid ap20187

Manufactured by Takara Bio
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The CID/AP20187 is a laboratory equipment designed for DNA/RNA sample preparation. It is a centrifugal device used for rapid and efficient nucleic acid extraction and purification. The core function of this product is to provide a convenient and standardized method for isolating genetic material from various sample types.

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Lab products found in correlation

P21 sc 271610, by Santa Cruz Biotechnology (2 mentions) Antibodies against lc3b 2775, by Cell Signaling Technology (2 mentions) Q vd oph, by Selleck Chemicals (1 mentions) Bortezomib, by Selleck Chemicals (1 mentions) Annexin 5, by BD (1 mentions) Facsverse instrument, by BD (1 mentions) 7 aad, by BD (1 mentions) Facsaria 2, by BD (1 mentions)

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AP20187 (53 citations)

5 protocols using cid ap20187

1

Targeted Cancer Signaling Modulation

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Alisertib, ABT263 and SBI0206965 were purchased from MedKoo. The chemical inducer of dimerization CID/AP20187 was purchased from Takara Bio. Antibodies against HSP90 (sc-13119), p53 (sc-126), and p21 (sc-271610) were purchased from Santa Cruz Biotechnology. Antibodies against LC3B (#2775), and BCL2 (#2872) were purchased from Cell Signalling.
Schepers A., Jochems F., Lieftink C., Wang L., Pogacar Z., Leite de Oliveira R., De Conti G., Beijersbergen R.L, & Bernards R. (2021). Identification of Autophagy-Related Genes as Targets for Senescence Induction Using a Customizable CRISPR-Based Suicide Switch Screen. Molecular Cancer Research, 19(10), 1613-1621.
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2

Characterization of Cell Death Pathways

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Alisertib, ABT263, and SBI0206965 were purchased from MedKoo. The chemical inducer of dimerization CID/AP20187 was purchased from Takara Bio. Antibodies against HSP90 (sc-13119), p53 (sc-126), and p21 (sc-271610) were purchased from Santa Cruz Biotechnology. Antibodies against LC3B (#2775) and BCL2 (#2872) were purchased from Cell Signalling Technology.
Schepers A., Jochems F., Lieftink C., Wang L., Pogacar Z., Leite de Oliveira R., De Conti G., Beijersbergen R.L, & Bernards R. (2021). Identification of Autophagy-Related Genes as Targets for Senescence Induction Using a Customizable CRISPR-Based Suicide Switch Screen. Molecular Cancer Research, 19(10), 1613-1621.
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3

Protein Dimerization and Apoptosis Inhibition

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CID/AP20187 was purchased from Clontech as B/B homodimerizer and diluted in 100% Ethanol. The proteasome inhibitor bortezomib and pan-caspase inhibitor qVD-Oph were purchased from Selleck Chemicals and R&D Systems, Inc., respectively and dissolved in DMSO.
Ando M., Hoyos V., Yagyu S., Tao W., Ramos C.A., Dotti G., Brenner M.K, & Bouchier-Hayes L. (2014). Bortezomib sensitizes non-small cell lung cancer to mesenchymal stromal cell-delivered inducible caspase-9-mediated cytotoxicity. Cancer gene therapy, 21(11), 472-482.
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4

Inducible Apoptosis in hiPSCs

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Eighty nmol/L CID/AP20187 (Clontech) was added to a dish containing NT-iC9-hiPSCs or a flask containing iC9-hiPSC-NS/PCs. Twenty-four hours after CID addition, the cells were stained with annexin V (BD Biosciences) and 7-AAD (BD Biosciences) for 15 min according to the manufacturer's instructions. Flow cytometry was performed using a FACS Verse instrument (BD Biosciences) with FlowJo software (Tree Star).
Itakura G., Kawabata S., Ando M., Nishiyama Y., Sugai K., Ozaki M., Iida T., Ookubo T., Kojima K., Kashiwagi R., Yasutake K., Nakauchi H., Miyoshi H., Nagoshi N., Kohyama J., Iwanami A., Matsumoto M., Nakamura M, & Okano H. (2017). Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives. Stem Cell Reports, 8(3), 673-684.
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5

Quantifying Apoptosis by Flow Cytometry

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CID/AP20187 was purchased from Clontech as a B/B homodimerizer. Cells that bound annexin V or took up 7-AAD were quantified by flow cytometry (FACSAria II; BD Biosciences) using FlowJo software (Tree Star). Details are provided in Supplemental Experimental Procedures.
Ando M., Nishimura T., Yamazaki S., Yamaguchi T., Kawana-Tachikawa A., Hayama T., Nakauchi Y., Ando J., Ota Y., Takahashi S., Nishimura K., Ohtaka M., Nakanishi M., Miles J.J., Burrows S.R., Brenner M.K, & Nakauchi H. (2015). A Safeguard System for Induced Pluripotent Stem Cell-Derived Rejuvenated T Cell Therapy. Stem Cell Reports, 5(4), 597-608.
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