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Calcium chloride cacl2

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Calcium chloride (CaCl2) is a chemical compound commonly used in various laboratory applications. It is a white, crystalline solid that is highly soluble in water. Calcium chloride serves as an essential electrolyte and is often used in applications that require the regulation of ionic balance or the maintenance of a specific pH level.

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Lab products found in correlation

Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Guar gum, by Merck Group (1 mentions) Acetaldehyde, by Thermo Fisher Scientific (1 mentions) 2 2 dimethoxy 2 phenylacetophenone dmpa, by Thermo Fisher Scientific (1 mentions) Sequencing grade trypsin, by Promega (1 mentions) Methyl α d mannopyranoside, by Merck Group (1 mentions) C18 omix tip, by Agilent Technologies (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Manganese dichloride, by Merck Group (1 mentions) Acetyl d18 glucosamine, by Merck Group (1 mentions) Ammonium bicarbonate abc, by Thermo Fisher Scientific (1 mentions) Panc 1 pancreatic ductal adenocarcinoma cells, by American Type Culture Collection (1 mentions) D lactose, by Merck Group (1 mentions) N methylmorpholine, by Merck Group (1 mentions) Triethylamine, by Merck Group (1 mentions) Dimethylformamide dmf, by Merck Group (1 mentions) Zirconium 4 chloride zrcl4, by Merck Group (1 mentions) Methanol ch3oh, by Merck Group (1 mentions) 1s 10 camphorsulfonic acid, by Merck Group (1 mentions) M phenylenediamine, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CaCl2 (168 citations) Calcium chloride (88 citations) Calcium chloride dihydrate (CaCl2·2H2O) (3 citations) Anhydrous calcium chloride (CaCl2) (2 citations)

14 protocols using calcium chloride cacl2

1

Synthesis and Characterization of Ferrofluid

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Commercial ferrofluid, EMG707, is purchased from Ferrotec Inc.(Nashua, NH USA). EMG707 ferrofluid is a water-based colloidal suspension containing Fe3O4 NPs (nominal particle diameter 10 nm) with a saturation magnetization of 110 Gauss. These EMG707 particles are synthesized via chemical co-precipitation26 27 using anionic dispersing agents (i.e. surfactants such as oleic acid) to stabilize the NPs against aggregation24 26 50 . The phosphate buffered saline solution (PBS) (BDH Chemicals) and cellulose acetate syringe filters (200 nm pore size) are purchased from VWR Inc. (Philadelphia, PA USA). Chemicals including calcium chloride (CaCl2) and sodium chloride (NaCl) are purchased from Fisher Scientific (Pittsburgh, PA USA).
Ye L., Pearson T., Cordeau Y., Mefford O.T, & Crawford T.M. (2016). Triggered self-assembly of magnetic nanoparticles. Scientific Reports, 6, 23145.
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2

Synthesis of Crosslinked Hydrogels

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Chemicals used in this work were acquired from different suppliers and used as received. Guar gum (product reference: G4129-250G), diallyl carbonate (CAR), 2,2′-(ethylenedioxy)diethanethiol (EDDET), trimethylolpropane tris(3-mercaptopropionate) (TriT), and methoxypolyethyleneglycol-350 (MeO-PEG-350) were acquired from Sigma-Aldrich (Madrid, Spain). Calcium chloride (CaCl2) was provided by Fisher Scientific (part of Thermo Fisher Scientific; Waltham, MA, USA). Alfa Aesar (part of Thermo Fisher Scientific; MA, USA) was the supplier of (2R,3R)-N,N′-diallyltartramide (TAR) and allyl alcohol. 2,2-Dimethoxy-2-phenylacetophenone (DMPA) and acetaldehyde were purchased from Acros Organics (part of Thermo Fisher Scientific; MA, USA).
Grosso R., Benito E., Carbajo-Gordillo A.I., García-Martín M.G., Perez-Puyana V., Sánchez-Cid P, & de-Paz M.V. (2023). Biodegradable Guar-Gum-Based Super-Porous Matrices for Gastroretentive Controlled Drug Release in the Treatment of Helicobacter pylori: A Proof of Concept. International Journal of Molecular Sciences, 24(3), 2281.
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3

Glycoproteomic analysis of PANC-1 cells

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Dithiothreitol (DTT), PNGase F and sequencing grade trypsin were from Promega (Madison, WI). Optimal LC/MS grade acetonitrile (ACN), methanol (MeOH) and water were from Fisher Scientific (Pittsburgh, PA). Concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinuscommunis agglutinin (RCA120), iodoacetamide (IAA), acetyl-D18 glucosamine, D-lactose, methyl α-D-mannopyranoside and manganese dichloride were obtained from Sigma-Aldrich (St. Louis, MO). Tris base, urea (UA), sodium chloride, ammonium bicarbonate (ABC) and calcium chloride (CaCl2) were obtained from Fisher Scientific (Pittsburgh, PA). Trifluoroacetic acid (TFA), triethylammonium bicarbonate (TEAB), N, N-dimethylformamide (DMF), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium tetrafluoroborate (DMTMM), N-methylmorpholine (NMM) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Hydroxylamine solution was purchased from Alfa Aesar (Ward Hill, MA). C18 OMIX tips were obtained from Agilent (Santa Clara, CA). Hydrophilic interaction chromatography material (PolyHYDROXYETHYL A) was obtained from PolyLC (Columbia, MD). Microcon filters YM-30 (30 kDa) was purchased from Merck Millipore (Billerica, MA). PANC-1 pancreatic ductal adenocarcinoma cells were from ATCC (Manassas, VA). Duplex DiLeu tags were custom synthesized in our own lab.45 (link)
Chen Z., Yu Q., Hao L., Liu F., Johnson J., Tian Z., Kao W.J., Xu W, & Li L. (2018). Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD). The Analyst, 143(11), 2508-2519.
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4

Synthesis of MOF-based Nanocomposites

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Zirconium (IV) chloride (ZrCl4, 99.5%), titanium (IV) isopropoxide (Ti [OCH(CH3)2]4, 97%), 1,4-benzenedicarboxylic acid (BDC, 98%), dimethylformamide (DMF, 99.9%), and methanol (CH3OH, 99.8%) were obtained from Sigma-Aldrich (St. Louis, MO, USA) for the preparation of MOFs NPs.
The PSU support sheets were prepared by dissolving polysulfone pellets (PSU, MW = 35,000, Sigma-Aldrich) in DMF solvent. The chemicals for the IP, m-phenylenediamine (MPD, ≥99%) and trimesoyl chloride (TMC, ≥98.5%), were purchased from Fisher Scientific (Pittsburgh, PA, USA) and Sigma Aldrich, respectively. 2,2,4-trimethylpentane (isooctane, 99%) was obtained from Fisher Scientific and used as TMC solvent. Our recent study demonstrated that isooctane could replace commonly used hexane during the IP process as a more environmental-friendly solvent [18 (link)]. Millipore DI water (Synergy185, 18.2 MΩ-cm, EMD Millipore Corp., Billerica, MA, USA) was used to prepare MPD solution and for cleaning purposes. Calcium chloride (CaCl2) and sodium chloride (NaCl) were obtained from Fisher Scientific and Sigma Aldrich, respectively. CSA/TEA salt materials, triethylamine (TEA, ≥99%) and (1s)-(+)-10-camphorsulfonic acid (CSA, 99%), were purchased from Sigma Aldrich.
Kadhom M., Hu W, & Deng B. (2017). Thin Film Nanocomposite Membrane Filled with Metal-Organic Frameworks UiO-66 and MIL-125 Nanoparticles for Water Desalination. Membranes, 7(2), 31.
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5

Preparation and Characterization of Daptomycin Lipid Vesicles

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Daptomycin was obtained from Cubist Pharmaceuticals, Inc. (Lexington,
Ma). The chemical structure of daptomycin is shown in Figure 1A. The phospholipids and lipid fluorophores
were purchased from Avanti Polar Lipids (Albaster, Al):
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-[1-rac-glycerol]
(POPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(POPC),
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[lissamine
rhodamine B sulfonyl] (LRh-DOPE, see Figure 1B), and 1-palmitoyl-2-(dipyrrometheneboron
difluoride)undecanoyl-sn-glycero-3-phospho-L-serine
(TopFluor-PS, see Figure 1C).
Carboxyfluorescein was purchased from Molecular Probes/Invitrogen (Carlsbad,
Ca). Sucrose and calcium chloride (CaCl2) were purchased from Fisher
Scientific (Pittsburg, Pa), and D(+)-glucose was purchased from ACROS
Organics (NJ).
Kreutzberger M.A., Pokorny A, & Almeida P.F. (2017). Daptomycin–Phosphatidylglycerol Domains in Lipid Membranes. Langmuir : the ACS journal of surfaces and colloids, 33(47), 13669-13679.
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6

Silica-Based Hybrid Nanoparticle Synthesis

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Polyethylenimine (PEI, average Mw = 25 kDa), tannic acid (1700 Da), poly(N-vinylpyrrolidone) (average Mw = 1300 kDa, PVPON-1300; and 58 kDa, PVPON-58), 46 wt % hydrofluoric acid, fluorescein isothiocyanate (FITC)-dextran, and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich. Alexa Fluor 488 succinimidyl ester was from Invitrogen. Porous silica (5- and 3-µm average sizes) and solid silica (2.0 ± 0.1 µm) microspheres were obtained from Restek and Polysciences, Inc., respectively. Doxorubicin hydrochloride (DOX) was purchased from LC Laboratories. Ultrapure deionized water (18.2 MΩ cm) was used for solution preparation (Evoqua). NaH2PO4 and Na2HPO4 buffers of ACS grade (Sigma-Aldrich) were used. Definity (Perflutren Lipid Microspheres) were obtained from Lantheus Medical Imaging, Inc. Poly(methacrylic acid) (PMAA; average Mw = 100 kDa), calcium chloride (CaCl2), and sodium carbonate (Na2CO3) were purchased from Fisher-Scientific. Fetal bovine serum (FBS, 10%) was purchased from Mediatech Inc.
Chen J., Ratnayaka S., Alford A., Kozlovskaya V., Liu F., Xue B., Hoyt K, & Kharlampieva E. (2017). Theranostic Multilayer Capsules for Ultrasound Imaging and Guided Drug Delivery. ACS nano, 11(3), 3135-3146.
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7

Synthesis and Characterization of Nanomaterials

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Magnesium sulfate (MgSO4), sucrose, sodium molybdate dihydrate (Na2MoO4·2H2O), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), gold (III) chloride trihydrate (HAuCl4·3H2O), sodium citrate tribasic dihydrate (C6H5Na3O7·2H2O), polyallylamine hydrochloride (PAH, MW 17.5 kDa), sodium borohydride (NaBH4), and sodium chloride (NaCl) were purchased from Sigma-Aldrich (Milwaukee, WI). Potassium phosphate dibasic trihydrate (K2HPO4·3H2O) was purchased from Mallinckrodt (Phillipsburg, NJ). Potassium phosphate monobasic (KH2PO4) was obtained from J.T. Baker (Center Valley, PA). Dulbecco’s phosphate-buffered saline was purchased from Corning (Aurora, CO), LB broth and agar were obtained from BD Difco (Franklin Lakes, NJ). SYTO9 nucleic acid stain was obtained from Molecular Probes (Waltham, MA). Calcium chloride (CaCl2) and ferrous sulfate (FeSO4) were purchased from Fisher Scientific (Rockford, IL). Absolute anhydrous 99.5% ethanol was obtained from Pharmco-Aaper (Brookfield, CT). All chemicals were used as received. Deionized water (18.2 MΩ) was purified using a Milli-Q Millipore water purification system (Billerica, MA).
Buchman J.T., Rahnamoun A., Landy K.M., Zhang X., Vartanian A.M., Jacob L.M., Murphy C.J., Hernandez R, & Haynes C.L. (2017). Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles. Environmental science. Nano, 5(2), 279-288.
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8

Alginate-Polymer Composite Hydrogel Synthesis

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Sodium sulfate (Na2SO4; anhydrate, purity
≥99.0%), sodium carbonate (Na2CO3), barium
nitrate [Ba(NO3)2;
dihydrate, purity ≥99.0%], alginic acid sodium salt [low viscosity,
4–12 cP, 1% in H2O (25 °C)], poly(ethylene
glycol) (MW 10,000 g/mol by GPC), poly(sodium 4-styrenesulfonate)
(PSS, MW 70,000 g/mol by GPC), poly(acrylic acid) sodium salt (MW
5100 g/mol by GPC), and d-(+)-gluconic acid δ-lactone
(GDL; C6H10O6) were purchased from
Sigma-Aldrich (St Louis, MO, USA). Calcium chloride (CaCl2; 96% purity, Acros Organics, USA), tetraethylene glycol (TEG; 99%,
Alfa Aesar, Thermo Fisher scientific, USA), and polyvinyl alcohol
(PVA, MW 30,000 g/mol, Merck KGaA, Germany) were used as supplied.
Stock aqueous solutions of 2.0% w/v alginate, used in most experiments,
were made by using alginic acid sodium salt powder with deionized
(DI) water. After complete dissolution, the aqueous alginate was stored
at 4 °C and was used within 1 week of mixing.
Kader M.S., Weyer C., Avila A., Stealey S., Sell S., Zustiak S.P., Buckner S., McBride-Gagyi S, & Jelliss P.A. (2022). Synthesis and Characterization of BaSO4–CaCO3–Alginate Nanocomposite Materials as Contrast Agents for Fine Vascular Imaging. ACS Materials Au, 2(3), 260-268.
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9

Antibody-based Protein Detection Protocol

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Anti-LAMP2 antibody was purchased from Santa Cruz Biotechnology (Dallas, USA). Polyclonal goat anti-mouse immunoglobulins HRP conjugated were purchased from Dako (Glostrup, Denmark). D-(+)-Galactose, zinc chloride (ZnCl2) and nickel sulfate hexahydrate (NiSO4) were purchased from Sigma (Saint Louis, USA), manganese (II) chloride tetrahydrate (MnCl2) was from Riedel-de-Haën (Seelze, Germany), calcium chloride (CaCl2) and iron (III) chloride (FeCl3) were from ACROS Organics (New Jersey, USA), copper (II) sulphate (CuSO4) was from Prolabo (France), lithium chloride (LiCl) was from Bio Basic Canada (Canada), magnesium chloride (MgCl2) was from Euromedex (Souffelweyersheim, France) and iron (II) tetrahydrate (FeCl2) was purchased from WVR Chemicals (Germany).
Vicogne D., Houdou M., Garat A., Climer L., Lupashin V., Morelle W, & Foulquier F. (2019). Fetal Bovine Serum impacts the observed N-glycosylation defects in TMEM165 KO HEK cells. Journal of inherited metabolic disease, 43(2), 357-366.
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10

Citrus Pectin Dissolution and pH Effects

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Citrus pectin with a degree of esterification of 35% (as specified by the provider) was kindly donated by Cargill. Calcium chloride (CaCl2) was from Acros Organics. Ethanol, potassium dihydrogen phosphate (KH2PO4), potassium hydroxide (KOH), sodium hydroxide (NaOH) and hydrochloric acid (HCl, 32%) were from Fisher Scientific. Theophylline was from Sigma Aldrich.
All products were used as received. Simulated gastric fluid (SGF) was prepared from HCl solution (0.1 N, pH 1.0) and simulated intestinal fluid (SIF) (pH 6.8) was made using NaOH at 11 mM (0.65 g/L) and KH2PO4 at 50 mM (6.8 g/L). Water was distillated.
Groult S., Buwalda S., & Budtova T. (2021). Pectin hydrogels, aerogels, cryogels and xerogels: Influence of drying on structural and release properties. European Polymer Journal, 149, 110386-110386.
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