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Cd135 apc pe cf594

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The CD135 APC & PE-CF594 is a lab equipment product that can be used to detect the presence of the CD135 cell surface marker. It contains two fluorescent-labeled antibodies, one conjugated with Allophycocyanin (APC) and the other with PE-CF594, which can bind to the CD135 antigen and enable its identification through flow cytometry or other analytical techniques.

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Close spelling variants of this product

CD135-PE CD135

2 protocols using cd135 apc pe cf594

1

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were kept at 4°C while staining in PBS with 0.5% BSA and 2 mM EDTA in the presence of CD16/32 block (BD clone 2.4G2).
The following antibodies were purchased from BD: CD45.1 BV711 (A20), CD117 BUV395 (2B8), CD135 APC & PE-CF594 (A2F10.1), MHC II V500, BV421, BV510 (M5/114.15.2), CD24 BUV496 (M1/69), CD127 BV421 (SB/199), CD45RA PE (14.8), CD19 BV42 1(1D3). From eBioscience: CD117 PE-Cy7 (2B8), CD317 eFluor450 & APC (eBio927), CD115 PE (AFS98), APC eFluor780 anti-CD11c (N418), MHC II eFluor450 (M5/114/15/2), CD24 PE-Cy7 (M1/69), CD172a APC & PerCP-eFluor710 (P84), Siglec-H PerCP-eFluor710 (eBio440C), eFluor450 Ter119 (Ter119), CD105 eFluor450 (MJ7/18), Irf8 PerCP-eFluor710 (V3GYWCH), CD45R eFluor450 (RA3-6B2), NK1.1 eFluor450 (PK136), CD3 eFluor450 (17A2), Irf4 PE (3E4). From Tonbo Biosciences: CD45.1 PE-Cy7 (A20), CD115 PE (AFS98), CD11c APC-Cy7 (N418). From BioLegend: CD115 BV711 (ASF98), CD45.1 Alexa Fluor 488 (A20), Flag (DYKDDDDK) APC (L5).
Lineage cell depletion kit was purchased from Miltenyi. Cells were analyzed on FACSCanto II or FACSAria Fusion flow cytometers (BD), and data analyzed with FlowJo software (Tree Star).
Grajales-Reyes G.E., Iwata A., Albring J., Wu X., Tussiwand R., Wumesh K., Kretzer N.M., Briseño C.G., Durai V., Bagadia P., Haldar M., Schönheit J., Rosenbauer F., Murphy T.L, & Murphy K.M. (2015). Batf3 maintains Irf8 autoactivation for commitment of a CD8α+ cDC clonogenic progenitor. Nature immunology, 16(7), 708-717.
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2

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were kept at 4°C while staining in PBS with 0.5% BSA and 2 mM EDTA in the presence of CD16/32 block (BD clone 2.4G2).
The following antibodies were purchased from BD: CD45.1 BV711 (A20), CD117 BUV395 (2B8), CD135 APC & PE-CF594 (A2F10.1), MHC II V500, BV421, BV510 (M5/114.15.2), CD24 BUV496 (M1/69), CD127 BV421 (SB/199), CD45RA PE (14.8), CD19 BV42 1(1D3). From eBioscience: CD117 PE-Cy7 (2B8), CD317 eFluor450 & APC (eBio927), CD115 PE (AFS98), APC eFluor780 anti-CD11c (N418), MHC II eFluor450 (M5/114/15/2), CD24 PE-Cy7 (M1/69), CD172a APC & PerCP-eFluor710 (P84), Siglec-H PerCP-eFluor710 (eBio440C), eFluor450 Ter119 (Ter119), CD105 eFluor450 (MJ7/18), Irf8 PerCP-eFluor710 (V3GYWCH), CD45R eFluor450 (RA3-6B2), NK1.1 eFluor450 (PK136), CD3 eFluor450 (17A2), Irf4 PE (3E4). From Tonbo Biosciences: CD45.1 PE-Cy7 (A20), CD115 PE (AFS98), CD11c APC-Cy7 (N418). From BioLegend: CD115 BV711 (ASF98), CD45.1 Alexa Fluor 488 (A20), Flag (DYKDDDDK) APC (L5).
Lineage cell depletion kit was purchased from Miltenyi. Cells were analyzed on FACSCanto II or FACSAria Fusion flow cytometers (BD), and data analyzed with FlowJo software (Tree Star).
Grajales-Reyes G.E., Iwata A., Albring J., Wu X., Tussiwand R., Wumesh K., Kretzer N.M., Briseño C.G., Durai V., Bagadia P., Haldar M., Schönheit J., Rosenbauer F., Murphy T.L, & Murphy K.M. (2015). Batf3 maintains Irf8 autoactivation for commitment of a CD8α+ cDC clonogenic progenitor. Nature immunology, 16(7), 708-717.
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