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Oregon green 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine og dhpe

Manufactured by Thermo Fisher Scientific

Oregon green 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (OG-DHPE) is a fluorescent lipid probe. It consists of a Oregon green fluorophore conjugated to a phosphoethanolamine headgroup and two long-chain fatty acid tails. This compound can be used to label and study lipid membranes and lipid-containing structures.

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2 protocols using oregon green 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine og dhpe

1

Lipid Characterization and Melting Points

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The following lipids were purchased from Avanti Polar Lipids (Alabaster, AL) in chloroform: DOPC, DPPC, DMPC, DiPhyPC, DGDG, SQDG, DOPG, and POPG. Texas red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (TX-DHPE) and Oregon green 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (OG-DHPE) were purchased from Thermo-Fisher Scientific. Cholestanol (chol) was procured from Sigma-Aldrich for use in ternary mixtures. Chain-melting temperatures for the lipids are as follows: DOPC (Tm≳−20 °C, ref. 36 (link)), DPPC (Tm≳41 °C, ref. 35 ), DMPC (Tm≳23 °C, ref. 35 ), DiPhyPC (Tm≳−120 °C, ref. 36 (link)), DGDG (Tm≳−50 °C, ref. 38 (link)), SQDG (Tm≳−10–20 °C, ref. 38 (link)), DOPG (Tm≳−18 °C, ref. 39 (link)), and POPG (Tm≳−5 °C, ref. 40 (link)).
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2

Sendai Virus Lipid Membrane Interaction

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Dioleoylphosphatidylethanolamine
(DOPE), palmitoyloleoylphosphatidylcholine (POPC), and cholesterol
(Chol), as well as ganglioside GD1a (from porcine brain), were purchased
from Avanti Polar Lipids (Alabaster, AL). Oregon Green-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (OG-DHPE) and Texas Red-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE) were obtained
from Thermo Fisher Scientific (Waltham, MA, U.S.A.). Polydimethylsiloxane
(PDMS) Sylgard 184 elastomer base and curing agent were purchased
from Ellsworth Adhesives (Germantown, WI, U.S.A.). Sendai virus (purified
Sendai Cantell Strain, egg-grown, batch 960216) was obtained from
Charles River Laboratories (Wilmington, MA, U.S.A.) and handled according
to a BSL-2 protocol at Williams College. Chloroform, methanol, and
buffer salts were obtained from Fisher Scientific (Pittsburgh, PA)
and Sigma-Aldrich (St. Louis, MO, U.S.A.). Formvar/carbon-coated square
mesh grids (Cu, 200 Mesh, SB) were purchased from Electron Microscopy
Sciences (Hatfield, PA, U.S.A.). Mouse anti-HN (1A6) IgG2a antibody
was purchased from Kerafast Inc. (Boston, MA) and produced in the
laboratory of Prof. Benhur Lee (Mt. Sinai). Characterization of the
1A6 antibody has been reported previously.10 (link),22 (link)
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