Cary 620

Manufactured by Agilent Technologies

The Cary 620 is a Fourier transform infrared (FTIR) imaging microscope designed for materials analysis. It provides high-resolution imaging and spectral data collection for a wide range of sample types, including polymers, composites, and biological materials.

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Lab products found in correlation

Cary 670, by Agilent Technologies (4 mentions) Resolutions pro, by Agilent Technologies (1 mentions) Cary 670 ftir spectrometer, by Agilent Technologies (1 mentions) Ti eclipse inverted optical microscope, by Nikon (1 mentions) Knowitall, by Bio-Rad (1 mentions) Cary 660, by Agilent Technologies (1 mentions) Freezone 2, by Labconco (1 mentions)

7 protocols using cary 620

FTIR Hyperspectral Imaging of Bone Osteons

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The bone sections were imaged by using a Fourier-transform infrared (FTIR) spectrometer (Agilent Cary 670) coupled with a microscope (Agilent Cary 620). The sections were measured in transmission mode by using a 15x-Cassegrain objective, an infrared radiation from a standard high-energy global middle-infrared light source and an infrared sensor, i.e. a liquid–nitrogen-cooled mercury-cadmium-telluride (MCT) focal-plane-array (FPA) detector consisted of 128 × 128 pixels of 1.1-μm² size. The sensor captures two-dimensional distributions of bone-transmission spectra in the infrared-wavenumber range from 3800 to 750 cm⁻¹ constituting hyperspectral images of 114-lp/mm optical resolution. The spectral resolution was 2 cm⁻¹, and the sensor’s integration time was 0.050 ms. The hyperspectral images were obtained from the 128-co-added-spectrum average of the same bone area to provide high-quality FTIR data and were acquired by using Resolutions Pro software provided with the Agilent Cary 620 FTIR Microscope. For each different osteon, a mosaic of four 128 × 128 pixel hyperspectral images were acquired, forming thus an FTIR-hyperspectral image of 282 × 282 μm field of view of the measured osteon. FTIR images (FTIR-hyperspectral images) of ten different osteons per cortical-bone section were captured. These studied osteons have osteoid whose the mean thickness is 30 μm ∓ 12 μm.

Palander A., Fauch L., Turunen M.J., Dekker H., Schulten E.A., Koistinen A., Bravenboer N, & Kullaa A. (2022). Molecular Quantity Variations in Human-Mandibular-Bone Osteoid. Calcified Tissue International, 111(6), 547-558.

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Molecular Characterization of RB Xenograft Tumors

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The FPA-FTIR study of the RB xenograft tumor samples was performed to identify the difference between the samples. FPA-FTIR micro-spectroscopic images were recorded using a Cary 620 FTIR microscope using cooled liquid-N2 environment with 128 × 128 element FPA detector objective lens, 15 × (0.62 NA) attached with FTIR spectrometer (Cary 670 FTIR spectrometer, Agilent Technologies). The FTIR Spectra were collected in a transmission mode in the spectral range between 3800-900 cm⁻¹. A single FTIR image was acquired in an area of 700 × 700 cm². A single FTIR spectral image contain the array of 64 × 64 spectra obtained from binning of the signal captured on detectors from each square of 4 on FPA array consist of 128 × 128 elements. A resultant single spectrum of sample collected in FTIR image acquired on ca. 10.9 × 10.9 mm² revealed the molecular information about the sample functional group. From each tumor sample 5 FTIR spectral images were obtained with a resolution of 4-cm⁻¹ with 128 co-added scans, Blackman-Harris 3-Term apodization, Power-Spectrum phase correction and a zero-filling factor of 2 using Resolutions Pro software package (Agilent Technologies). Before each sample measurement, background measurements were performed using a clean surface of the substrate with the same acquisition parameters17 (link).

Kalmodia S., Parameswaran S., Yang W., Barrow C.J, & Krishnakumar S. (2015). Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy: An analytical technique to understand therapeutic responses at the molecular level. Scientific Reports, 5, 16649.

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FTIR and Dark-field Imaging of Breast and Ovarian Tissue

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Both FTIR and dark-field microscopy are used to image unstained tissue cores from breast and ovarian TMAs. Tissue sections were prepared using standard protocols^{11 (link)} for FTIR imaging. 5 μm thick tissue sections from FFPE blocksmounted on IR transparent windows of CaF₂ were deparaffinized for imaging, first imaged with FTIR imaging system (Agilent 670 spectrometer coupled to a Cary 620 microscopy system) and then with a dark-field microscope (Nikon Eclipse Ti inverted optical microscope). Agilent Cary 620 FTIR has 15 × 0.62NA and 128 × 128 pixels focal plane array (FPA) detector. We collected mid-IR HS images of tissue sections using standarddefinition (SD) mode with 5.5 μm pixel size and 8 cm⁻¹ spectral resolution in the spectral range of 1000 to 3900 cm⁻¹.
Tissue sections were imaged with a Nikon inverted optical microscope with a 10×, 0.4NA objective in the dark-field mode. A dark-field condenser transmits a hollow cone of light and blocks light from within a disk around the optical axis. In the presence of a sample, scattered light is collected by the objective forming a bright image against a dark background. Based on the Rayleigh criteria, the diffraction-limited spatial resolution of dark-field images collected in the visible range (400 to 700 nm) is significantly higher than FTIR images in the fingerprint region (2.5 to 12 μm).

Mankar R., Gajjela C.C., Shahraki F.F., Prasad S., Mayerich D, & Reddy R. (2021). Multi-modal image sharpening in fourier transform infrared (FTIR) microscopy. The Analyst, 146(15), 4822-4834.

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Microplastic Identification via FTIR

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Suspected MPs were analysed using a Cary 670 FTIR spectrometer equipped with a Cary 620 microscope (Agilent Technologies, Mulgrave, AUS). Particles, including individual microfibres, were analysed using the micro-ATR accessory equipped with a Germanium crystal. For this, individual particles were affixed to a glass microscope slide covered with a thin layer of 2% dextrose (Sigma-Aldrich, St. Louis, USA) using microtweezers. For each sample, 128 co-added scans at a resolution of 8 cm⁻¹ in the range of 3800–900 cm⁻¹ were collected. The spectra were matched against commercial library of FTIR spectra (KnowItAll, Bio-Rad). Sample spectra were identified successfully if they met the following criteria: (i) all major peaks were present in both reference and sample spectra and (ii) the total overlap of the reference and sample spectra was >80%.

Ross P.S., Chastain S., Vassilenko E., Etemadifar A., Zimmermann S., Quesnel S.A., Eert J., Solomon E., Patankar S., Posacka A.M, & Williams B. (2021). Pervasive distribution of polyester fibres in the Arctic Ocean is driven by Atlantic inputs. Nature Communications, 12, 106.

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ATR-FTIR Spectromicroscopy Chemical Imaging

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ATR–FTIR spectromicroscopy with chemical imaging was performed at the Heritage Conservation Centre (HCC), Singapore. The Agilent Cary 670 FTIR spectrometer and Cary 620 microscope system consists of a 15 X objective (NA = 0.62) and a 64 × 64 focal plane array (FPA) detector. With a micro-Ge (germanium) ATR inserted at the microscope objective, this configuration gave a field of view of about 70 × 70 µm (1.1 µm² pixels) and the simultaneous acquisition of 4096 spectra (in seconds to minutes).
The sample was placed on a motorised stage with a micro-vice and gently brought into sufficient contact with the ATR crystal. The pressure applied to the sample was adjusted to optimise signal intensity. The spectra were collected between 4000 and 900 cm⁻¹ with the co-addition of 64 scans at 4 cm⁻¹ resolution. FTIR chemical images of the sample were obtained by selecting the integrated absorbance of the characteristic wavenumbers of interest.

Chua L., Banas A, & Banas K. (2022). Comparison of ATR–FTIR and O-PTIR Imaging Techniques for the Characterisation of Zinc-Type Degradation Products in a Paint Cross-Section. Molecules, 27(19), 6301.

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Characterization of Hook Nanoantenna Spectral Response

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A Fourier-transformed IR (FTIR) microscope (Agilent Cary 660) with an FTIR spectrometer (Agilent Cary 620) and liquid-nitrogen-cooled HgCdTe (mercury cadmium telluride, MCT) detector is used to characterize the spectral response of hook nanoantenna. The background signal is collected from the CaF₂ chip using 16-32 scans at 8 cm⁻¹ resolution to compensate for the MIR gas absorption (mainly water vapor and CO₂) from the ambient. Then the sample scan is performed using 16-32 scans at 8 cm⁻¹ resolution to capture the spectral response of nanoantenna. The scanning area is adjusted to 200*200 μm² to fit the nanoantenna area. For liquid sensing, a microfluidic chamber made by PDMS is bonded to a CaF₂ chip to allow the contact of the liquid analyte with HNA, and the spectrum is captured simultaneously.

Ren Z., Zhang Z., Wei J., Dong B, & Lee C. (2022). Wavelength-multiplexed hook nanoantennas for machine learning enabled mid-infrared spectroscopy. Nature Communications, 13, 3859.

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ATR-FTIR Analysis of Bilayer Tablets

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Attenuated total-reflection FTIR spectroscopy (Agilent Technologies) was performed in the range of 500–4,000 cm⁻¹ using a Cary670 (main bench) and Cary620 (microscope). FTIR spectra of drug, excipients, and physical mixture in each layer were obtained and peaks that could trace the drug molecule identified. To confirm the effect of freeze-drying, physical mixtures of each layer were also evaluated after freeze-drying by FTIR. FTIR imaging was performed with the same instrument.17 (link),33 (link),34 (link) After dissolution for predetermined times — 30, 240, and 480 minutes for both layers — bilayer tablets were removed from dissolution vessels, frozen at −75°C in a deep-freezer for 24 hours, then lyophilized at −50°C, 0.07 mBar for 72 hours with a freeze-dryer (FreeZone 2.5; Labconco). After freeze-drying, bilayer tablet was cut vertically down the center to observe the cross section. The outline of the cross-sectioned layer was observed under an IR field of view of 325×325 and IR pixel size of 5.5×5.5. Each image was analyzed using ImageJ (version 1.51j8) with Color Inspector 3-D version 2.0 (Internationale Medieninformatik, Berlin, Germany) plug-ins and three-dimensional color space was produced, giving an 8-bit red–green–blue value for each image to easily identify the distribution of each drug molecule.

Lee H.G., Park Y.S., Jeong J.H., Kwon Y.B., Shin D.H., Kim J.Y., Rhee Y.S., Park E.S., Kim D.W, & Park C.W. (2019). Physicochemical properties and drug-release mechanisms of dual-release bilayer tablet containing mirabegron and fesoterodine fumarate. Drug Design, Development and Therapy, 13, 2459-2474.

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