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Recombinant human pdgf bb

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Recombinant human PDGF-BB is a purified protein produced in a recombinant system. It is a platelet-derived growth factor composed of two B subunits.

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Lab products found in correlation

Ly294002, by Merck Group (1 mentions) Sb415286, by Merck Group (1 mentions) Proliferating cell nuclear antigen antibody, by Cell Signaling Technology (1 mentions) Fetal bovine serum fbs, by PromoCell (1 mentions) Trypan blue reagent, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem f12, by Thermo Fisher Scientific (1 mentions) α sma, by Abcam (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Phospho erk, by Abcam (1 mentions) Total erk, by Abcam (1 mentions) Desmin, by Abcam (1 mentions) Total akt, by Abcam (1 mentions) Smoothelin, by Abcam (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Abcam (1 mentions) Mouse anti cyclin d1, by Abcam (1 mentions) Anti mouse and rabbit horseradish peroxidase conjugated secondary antibodies, by Merck Group (1 mentions)

Close spelling variants of this product

PDGF-BB (94 citations) Human PDGF-BB (3 citations)

3 protocols using recombinant human pdgf bb

1

Investigating PDGF-BB-Mediated Cell Proliferation

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Recombinant human PDGF-BB, trypan blue reagent, the phosphoinositide 3-kinase (PI3K) specific inhibitor, LY294002, the GSK-3β antagonist, SB415286, and cell proliferation reagent 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma, St. Louis, MO, USA. The proliferating cell nuclear antigen (PCNA) antibody was purchased from Cell Signaling Technology (Product no. 2586s). Trypsin-ethylenediaminetetraacetic acid (EDTA) (0.25%), Dulbecco's modified Eagle's medium/F12 (DMEM/F12) and fetal bovine serum (FBS) were from PromoCell (Heidelberg, Germany). The digoxin injection was acquired from Minsheng Pharmaceutical Group Co., Ltd. (Hangzhou, China). Digoxin was purchased from J&K Scientific Ltd. (Beijing, China) and dissolved in dimethyl sulfoxide (DMSO), and the concentration of DMSO was <0.8% in the control and drug-containing medium.
YAN G., WANG Q., HU S., WANG D., QIAO Y., MA G., TANG C, & GU Y. (2015). Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury. International Journal of Molecular Medicine, 36(4), 1001-1011.
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2

Smooth Muscle Cell Proliferation Assay

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PRF was obtained from Anhui Joyfar Pharmaceutical Co., Ltd, (Bozhou, China), while Dulbecco’s modified Eagle’s medium (DMEM)/F12 and fetal bovine serum (FBS) were purchased from Life Technologies (Carlsbad, CA, USA). Dimethyl sulfoxide (DMSO), MTT and recombinant human PDGF-BB were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse anti-cyclin D1, proliferating cell nuclear antigen (PCNA), cyclin-dependent kinase (CDK) 4, α-SMA, desmin, smoothelin, phospho-protein kinase B (Akt), total Akt, phospho-ERK, total ERK, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies and rabbit anti-mouse secondary antibodies were obtained from Abcam (Cambridge, UK).
LI H., LUO K, & HOU J. (2014). Inhibitory effect of Puerariae radix flavones on platelet-derived growth factor-BB-induced proliferation of vascular smooth muscle cells via PI3K and ERK pathways. Experimental and Therapeutic Medicine, 9(1), 257-261.
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3

Phospho-Protein Detection Assay

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Rabbit phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® (#4511), rabbit phospho-SAPK/JNK (Thr183/Tyr185) (81E11) (#4668), rabbit phospho-cjun (Ser73) (D47G9) XP® (#3270) antibodies were from Cell Signaling Technology. Mouse GAPDH (#MAB374) as well as anti-mouse and rabbit horseradish peroxidase-conjugated secondary antibodies (#401253 and #401353) were from Merck Millipore. Mouse PSCD2 (ARNO) clone 6H5 antibody was from Sigma-Aldrich, and goat GRP1 (cytohesin-3) (C-20) (sc-9732) and goat cytohesin-1 (N-19) (sc-9733) antibodies were from Santa Cruz. Inhibitors for AP-1 (SR11302), JNK (SP600125), p38 MAPK (SB203580) were from Tocris. Alexa-Fluor 546 labelled Phalloidin was from Molecular Probes. Recombinant human Resistin (#RD 172016301) was from BioVendor. Recombinant human PDGF-BB (#3201) and all other chemicals were from Sigma-Aldrich.
Heun Y., Gräff P., Lagara A., Schelhorn R., Mettler R., Pohl U, & Mannell H. (2020). The GEF Cytohesin-2/ARNO Mediates Resistin induced Phenotypic Switching in Vascular Smooth Muscle Cells. Scientific Reports, 10, 3672.
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