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2 protocols using rabbit anti p105

1

Immunoblotting of Signaling Pathways

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THP1 cells were lyzed in 1X SDS-PAGE loading buffer with protease and phosphatase inhibitor cocktails (Roche). Samples were resolved on a 4%−20% gradient SDS-PAGE gel. Following transfer of the proteins, the nitrocellulose membrane was blocked in 5% milk for 1h and probed with the following antibodies overnight at 4°C: goat anti-IFIT1 (Santa Cruz, 82946), Rabbit anti-phos-pho-p38 (Cell Signaling, 4511S), Rabbit anti-phospho-p65 (Cell Signaling, 3033S), Rabbit anti-p105 (Santa Cruz, sc293141), Mouse anti-GAPDH (Cell Signaling, 97166), Rabbit anti-hnRNPL (Santa Cruz, sc-32317), Rabbit anti-phospho-IRF3 (Abcam, ab76493), Rabbit anti-phospho-JNK (Cell Signaling, 9255), Rabbit anti-phospho-ERK (Cell Signaling, 4370), Rabbit anti-phospho-STAT1 (Cell Signaling, 9167S). Western blots were incubated with respective HRP-conjugated secondary antibodies and visualized using ECL reagents (Pierce).
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2

Immunoblotting of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
THP1 cells were lyzed in 1X SDS-PAGE loading buffer with protease and phosphatase inhibitor cocktails (Roche). Samples were resolved on a 4%−20% gradient SDS-PAGE gel. Following transfer of the proteins, the nitrocellulose membrane was blocked in 5% milk for 1h and probed with the following antibodies overnight at 4°C: goat anti-IFIT1 (Santa Cruz, 82946), Rabbit anti-phos-pho-p38 (Cell Signaling, 4511S), Rabbit anti-phospho-p65 (Cell Signaling, 3033S), Rabbit anti-p105 (Santa Cruz, sc293141), Mouse anti-GAPDH (Cell Signaling, 97166), Rabbit anti-hnRNPL (Santa Cruz, sc-32317), Rabbit anti-phospho-IRF3 (Abcam, ab76493), Rabbit anti-phospho-JNK (Cell Signaling, 9255), Rabbit anti-phospho-ERK (Cell Signaling, 4370), Rabbit anti-phospho-STAT1 (Cell Signaling, 9167S). Western blots were incubated with respective HRP-conjugated secondary antibodies and visualized using ECL reagents (Pierce).
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