The tissue samples underwent lysis in SDS‐buffer and separation in polyacrylamide‐sodium dodecyl sulfate (SDS) gel and were electrotransferred to nitrocellulose membranes. The membranes were then probed with rabbit anti‐vWF (Abcam) or mouse anti‐Actin antibody (BD Bioscience). The membranes were incubated with the respective horseradish peroxidase‐conjugated antirabbit or antimouse immunoglobulin G secondary antibodies (R&D Systems). Antibody binding was visualized using the SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific). The quantification was performed by densitometric measurement using ImageJ software.
Mouse anti actin antibody
Manufactured by BD
The Mouse anti-Actin antibody is a laboratory tool used to detect and quantify the presence of the actin protein in biological samples. Actin is a highly abundant and ubiquitous cytoskeletal protein found in all eukaryotic cells. This antibody specifically binds to the actin protein, allowing researchers to study its expression and distribution in various cell types and tissues.
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Lab products found in correlation
2 protocols using mouse anti actin antibody
1
Western Blot Analysis of vWF and Actin
2
Antibody Production for AMPA Receptor Subunits
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Rabbit polyclonal antibodies against AMPA receptor subunits were raised against synthetic peptides with the following sequences: anti-GluA1–3, (C)VNLAVLKLSEQGVLDKLKSKWWYDKGE (residues 760–786 of mouse GluA1), anti-GluA1, (C)SMSHSSGMPLGATGL (residues 893–907 in the C-terminal intracellular region of rat GluA1), anti-GluA3, (C)NEYERFVPFSDQQIS (residues 394–408 in the N-terminal extracellular region of rat GluA3), and anti-GluA4, (C)GTAIRQSSGLAVIASDLP (residues 885–902 in the C-terminal intracellular region of rat GluA4). Mouse monoclonal anti-GluA2 was raised against the synthetic peptide (C)YKEGYNVYGIESVKI (residues 869–883 in the C-terminal intracellular region of rat GluA2). Affinity-purified antibodies were obtained from antisera using respective antigen peptides. Mouse anti-actin antibody was obtained from BD Biosciences.
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