Lamp1 mcherry
LAMP1-mCherry is a plasmid that encodes the lysosome-associated membrane protein 1 (LAMP1) fused to the mCherry fluorescent protein. LAMP1 is a widely used marker for lysosomes, and the mCherry tag allows for the visualization of LAMP1-positive organelles.
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6 protocols using lamp1 mcherry
Polycation-Mediated Gene Delivery System
Transfection and Silencing Protocols for Neuronal Proteins
Lentiviral vectors expressing a siRNA cassette and GFP reporter were originally described in Wiznerowicz and Trono (2003) (link). The mouse Negr1 and mouse Fgfr2 silencing lentiviral vectors used in biochemical assays (siNegr1 and siFGFR2) and in utero electroporation experiments (siNegr1) are described in Pischedda and Piccoli (2015) (link). The FGFR2 silencing vectors used in in utero electroporation experiments (siRNA 2A: 5′-GCACACACTTACAGAGCACAA-3′ targeting mouse Fgfr2 3′UTR and siRNA 2B: 5′-CCTCTCTACGTCATAGTTGAA-3′ targeting mouse Fgfr2 exon 11) were originally described in Tarkkonen et al. (2012) (link). The silencing cassettes, including a U6 promoter, and silencing sequences 2A and 2B were also cloned into a pDsRed-expression vector (Clontech).
Overexpression and Silencing of STIM1 in Fibroblasts
Plasmid Transfection and Autophagy Monitoring
Tracking Vesicle Dynamics in 2D and 3D Cultures
Immunocytochemistry Visualization of P2RX4 and LAMP1
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