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A 100 bp dna size marker

Manufactured by Takara Bio
Sourced in Japan

A 100-bp DNA size marker is a laboratory tool used to determine the size of DNA fragments. It provides a reference scale to estimate the size of unknown DNA samples during gel electrophoresis. The marker consists of a mixture of DNA fragments of known sizes, typically ranging from 100 to 3,000 base pairs, in increments of 100 base pairs. This allows for accurate sizing of DNA samples by comparing their migration patterns to the known sizes of the marker fragments.

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2 protocols using a 100 bp dna size marker

1

PMMA-PDMS Microfluidic Device Fabrication

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PMMA sheets (2 mm thick) were purchased from Goodfellow (Huntingdon, England). PDMS prepolymer (Sylgard 184) and a curing agent were purchased from Dow Corning (Midland, MI, USA). 3-Aminopropyltriethoxysilane (APTES, 99%), monoglycidyl ether terminated PDMS (average Mn 5,000 g/mol), and bovine serum albumin (BSA; V fraction) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Taq polymerase, PCR buffer solutions, deoxynucleotides (dNTPs), and genomic DNA purification kit were purchased from Promega (Madison, WI, USA). Luria-Bertani (LB) broth was purchased from MB cell (Los Angeles, CA, USA), and trypticase soy broth was purchased from Becton Dickinson (Franklin Lakes, NJ, USA). A 100-bp DNA size marker was purchased from Takara (Shiga, Japan). Agarose powder was purchased from BioShop (Burlington, ON, Canada). Ethidium bromide dye (Loading STAR) used for staining DNA was purchased from Dynebio (Seongnam, Korea). Robotic QIAcube workstation was purchased from Qiagen (Germantown, MD, USA).
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2

Surface Functionalization of Polymers

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(3-Aminopropyl)triethoxysilane (APTES; 99%) and [2-(3,4-epoxycyclohexyl)ethyl]trimethoxysilane (ECTMS; 98%) were purchased from Sigma-Aldrich. The PDMS prepolymer (Sylgard 184) and curing agent were obtained from Dow Corning. Poly(methyl methacrylate) (PMMA), polycarbonate (PC), polystyrene (PS), and polyethylene terephthalate (PET) were purchased from Goodfellow. Fluospheres amine (0.2 μm, red) and TE buffer (10 nM Tris–HCl, 0.1 nM EDTA, pH 8.0) were obtained from Thermo Fisher Scientific. Taq polymerase, PCR buffer solutions, and dNTPs were obtained from BioFact (Daejeon, Korea). Agarose powder was purchased from BioShop (Ontario, Canada). A 100 bp DNA size marker was purchased from Takara (Shiga, Japan). Ethidium bromide (EtBr)-based dye (Loading STAR) was purchased from Dynebio (Seongnam, Korea).
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