Lung tissue sections were prepared as previously described with some modifications (23 ). Briefly, lung tissues were fixed in 10% neutral phosphate buffered formalin for 24 hours at room temperature. After paraffin processing (TEK VIP; Sakura Finetek; Torrance, CA, USA) and embedding (EG1160; Leica Biosystems; Buffalo Grove, IL, USA), paraffin microtomy (RM2135; Leica Biosystems) was performed at 5 microns thickness of slide for each section. Three sections were processed with 50 microns between each. For each biopsy core, three consecutive sections are placed on 3 slides (total of 9 sections per lung tissue) to assure detection of tumorigenesis in a whole lung. Slides of sections were deparaffinized and rehydrated in xylene, ethanol and deionized water. Slides were stained in hematoxylin (95057-844; VWR; Radnor, PA, USA). After rinsing with deionized water, the slides were stained by eosin (HT110232; Thermo Fisher) and dehydrated in ethanol and xylene. Coverslips were mounted on slides by xylene-based permountTM mounting medium (SP15-500; Thermo Fisher). Finally, the slides were dried overnight in a chemical hood. The slides were scanned by Aperio Imagescope (Leica Biosystems) and analyzed with the software (version 12.3.3).
Ht110232
Manufactured by Thermo Fisher Scientific
The HT110232 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform specific functions within a laboratory setting. The core function of this equipment is to provide a means for conducting various scientific experiments and analyses, but a detailed description of its intended use or operation is not available at this time.
Automatically generated - may contain errors
2 protocols using ht110232
1
Lung Tissue Histological Processing and Imaging
2
Quantitative Lung Tumor Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lung tissue sections were prepared as previously described, with some modifications52 . Briefly, lung tissues were fixed in 10% neutral phosphate buffered formalin for 24 h at room temperature. After paraffin processing (TEK VIP; Sakura Finetek; Torrance, CA) and embedding (EG1160; Leica Biosystems; Buffalo Grove, IL), paraffin microtomy (RM2135; Leica Biosystems) was performed at 5 μM thickness per section. 5 sections were processed with 200 μM between each to assure the detection of tumorigenesis in a whole lung. Slides of sections were deparaffinized and rehydrated in xylene, ethanol, and deionized water. Slides were stained with hematoxalin (95057-844; VWR) and eosin (HT110232; Thermo Fisher) according to a published protocol52 . Stained slides were dehydrated in ethanol and xylene and coverslips were affixed to slide using xylene-based Permount™ mounting medium (SP15-500; Thermo Fisher; Waltham, MA). Slides were dried overnight in a chemical hood, scanned using an Aperio Imagescope (Leica Biosystems). CaseViewer software (3DHISTECH; Ramsey, NJ) was used to outline and measure lung/tumor cross-sectional area. Tumor burden was calculated for all sections as the sum of tumor cross-sectional area divided by the cross-sectional area of the whole lung section.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!