1H and 13C NMR spectra were on a Varian INOVA 500-MHz NMR spectrometer using CDCl3 as solvent. Gel permeation chromatography (SEC) was performed on an Agilent 1260 Infinity equipped with a PLgel 5-μm MIXED-D column and differential refractive index detectors. THF was used as an eluent with a flow rate of 0.3 ml min−1 at 35°C. A polystyrene standard kit (Agilent Technologies) was used for calibration. Automated column chromatography was performed on a Biotage Selekt flash chromatography purification system equipped with a Sfär silica column cartridge. n-Hexane and ethyl acetate were used as eluent. SEC was performed on a Recycling Preparative HPLC (high-performance liquid chromatography) (LC-9260 NEXT, Japan Analytical Industry) system equipped with JAIGEL-2.5HR/2HR columns and a differential refractometer. Chloroform (J.T.Baker) was used as an eluent with a flow rate of 10 ml min−1. Before injection, the solution was filtered through a polytetrafluoroethylene syringe filter (Whatman; 0.2-μm pore). The SEC was performed under a cycling mode until the coinciding peaks were separated. The desired fraction was collected using a fraction collector.
Polytetrafluoroethylene syringe filter
Manufactured by Cytiva
Sourced in United Kingdom
The Polytetrafluoroethylene (PTFE) syringe filter is a laboratory filtration device used to remove particulate matter from liquid samples. It features a PTFE membrane that allows the liquid to pass through while trapping solid contaminants. The filter is designed for single-use and is typically used in sample preparation, analytical procedures, and various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Whatman, by Cytiva (4 mentions)
5 μm mixed d column, by Agilent Technologies (1 mentions)
1260 infinity, by Agilent Technologies (1 mentions)
Inova 500 mhz nmr spectrometer, by Agilent Technologies (1 mentions)
Acetic acid solution, by Merck Group (1 mentions)
Bovine milk, by Merck Group (1 mentions)
4 protocols using polytetrafluoroethylene syringe filter
1
Characterization of Polymeric Materials
2
Biocompatible Chitosan Solution Preparation
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For the preparation of a biocompatible chitosan solution, a mixture of chitosan powder derived from shrimp shells and acetic acid solution was used. Specifically, 2 wt% chitosan powder was added to a 2 wt% acetic acid solution, which was then diluted with 10 mL of deionized water. The mixture was stirred constantly at 800 rpm for 6 h at 50 °C using a magnetic stirrer until the powder entirely dissolved. To remove various impurities, the solution was filtered using a polytetrafluoroethylene syringe filter with a pore size of 5 μm (Whatman International Ltd., Maidstone, UK).
3
Fabrication of Chitosan Electrolyte Solution
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Chitosan electrolyte solution, which plays an important role in mimicking synaptic behaviors, was prepared as follows. First, 2 wt% chitosan powder (deacetylation degree > 75%) was added to deionized water diluted with 2 wt% acetic acid (purity > 99%). The solution was then completely dissolved by magnetic stirring at 800 rpm for 6 h at 50 °C. Finally, the chitosan electrolyte solution was obtained by filtering the solution using a polytetrafluoroethylene syringe filter with a 5 μm pore size (Whatman International Ltd., Maidstone, UK).
4
Biocompatible Casein Solution Preparation
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A biocompatible casein solution was prepared by dissolving a mixture of casein powder and acetic acid solution. Herein, 3 wt% casein powder derived from bovine milk (technical grade, Sigma-Aldrich) was added to a 3 wt% acetic acid solution with purity > 99% (Sigma-Aldrich) and diluted with deionized water. The solution was then constantly mixed and completely dissolved using a magnetic stirrer with 800 rpm for 6 h at 130 °C. Finally, the solution was filtered through a polytetrafluoroethylene syringe filter with a 5 μm pore size (Whatman International Ltd., Maidstone, UK) to eliminate impurities. Figure 1 a depicts the prepared casein solution.
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