The hUCMSCs cells were grown in T-75 cell culture flask (Falcon, Italy) at early passages (1–6), in complete culture medium MEM, supplemented with 10% FBS and antibiotics (penicillin G sodium 100 U/mL, streptomycin 100 U/mL), in a humidified and controlled atmosphere at 37 °C and 5% CO2. The medium was changed every 3–4 days. When confluent growth was reached, the cells were detached with 0.25% trypsin—EDTA solution and washed twice with PBS. The resulting cell suspensions were centrifuged (5 min, 1000 rpm; BRK55/10 Centrifuge by Centurion Scientific Ltd., Stoughton, UK), the supernatant separated, and the cells re-suspended in fresh culture medium. Viable cells were counted using the TC20 automated Cell Counter (Biorad, Hercules, CA, USA).
T 75 cell culture flask
Manufactured by BD
Sourced in Italy
The T-75 cell culture flask is a laboratory equipment designed for the culturing of cells. It provides a surface area of 75 cm² for cell growth and proliferation. The flask is made of transparent polystyrene material, allowing for visual inspection of the cell culture.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using t 75 cell culture flask
1
Expansion and Characterization of hUCMSCs
2
Evaluating L929 Cell Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To evaluate the biological
response of the formulations, L929 cells derived from mouse C34/An
connective tissues (Sigma-Aldrich, USA) were used. L929 cells were
grown in a T-75 cell culture flask (Falcon, Italy), in cell culture
medium Dulbecco’s Modified Eagle’s medium (Hyclone,
USA) supplemented with 10% fetal bovine serum and antibiotics (penicillin
G sodium 100 U/mL, streptomycin 100 μg/mL) at 37 °C and
5% CO2. The medium was changed every 3–4 days.
response of the formulations, L929 cells derived from mouse C34/An
connective tissues (Sigma-Aldrich, USA) were used. L929 cells were
grown in a T-75 cell culture flask (Falcon, Italy), in cell culture
medium Dulbecco’s Modified Eagle’s medium (Hyclone,
USA) supplemented with 10% fetal bovine serum and antibiotics (penicillin
G sodium 100 U/mL, streptomycin 100 μg/mL) at 37 °C and
5% CO2. The medium was changed every 3–4 days.
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