DNA synthesis was quantified employing the 5-bromo-2’-deoxyuridine (BrdU) labelling and detection enzyme-linked immunosorbent assay kit (Roche Diagnostics, Mannheim, Germany). Therefore, quiescent or activated (proliferating) PSCs were plated in 96-well plates at equal seeding densities and allowed to adhere overnight. Afterwards, the cells were exposed to vitamin D2, vitamin D3 (both from Santa Cruz Biotechnologies, Heidelberg, Germany) or calcipotriol (Sigma-Aldrich, Deisenhofen, Germany) for the indicated periods of time. Twenty-four hours prior to cell harvesting, BrdU labelling was initiated by adding labelling solution at a final concentration of 10 μmol/L. Afterwards, labelling was stopped, and BrdU uptake was measured according to the manufacturer’s instructions.
Vitamin d3
Manufactured by Santa Cruz Biotechnology
Sourced in Germany, Poland
Vitamin D3 is a fat-soluble vitamin that is essential for the maintenance of normal calcium and phosphorus levels in the body. It plays a crucial role in the absorption and utilization of calcium, which is necessary for the development and maintenance of healthy bones and teeth.
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Lab products found in correlation
2 protocols using vitamin d3
1
Quantifying Cell Proliferation using BrdU Assay
2
Effect of Vitamin K and D3 on Osteoblasts
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To evaluate the in vitro effect of vitamin K (K1, MK4, and MK7) and vitamin D3 on osteoblasts’ metabolism cultured with biomaterial cells were cultured on commercial biomaterials of different origin and chemical composition. Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells. Four different hydroxyapatites: human origin (Maxgraft, Botiss biomaterials GmgH, Germany), bovine origin (Cerabone, Botiss Biomaterials GmbH, Germany), porcine origin (Apatos, Tecnoss, Italy), and porcine hydroxyapatite with preserved collagen (Gen-Os, Tecnoss, Italy) were used for experiments.
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