Clone 11b11

T cells were differentiated as described before^{56 (link),57 (link)}. In brief, T cells were isolated from spleen and lymph nodes of WT mice. CD4⁺ T cells were purified using the Naive CD4⁺ T Cell Isolation Kit (Miltenyi, no. 130104453). Naive cells were cultured at a concentration of 1.5–2.0 × 10⁶ per ml. Cells were seeded in the culture plates pre-coated with anti-CD3 antibody (2 μg ml⁻¹ for effector T cells, 0.5 µg ml⁻¹ for regulatory T cells) and anti-CD28 antibody (2 μg ml⁻¹) (clone PV-1; all from Bio X Cell). For the differentiation of T_H17 cells, naive T cells were cultured with IL-6 (30 ng ml⁻¹, R&D, no. R&D406-ML), TGF-β (3 ng ml⁻¹, R&D, no. R&D 240-B) and anti-IFN-γ (10 µg ml⁻¹, clone XMG1.2, BioXCell, no. BE0055), for T_H1 with IL-12 (10 ng ml⁻¹), and anti-IL-4 (10 µg ml⁻¹, clone 11B11, BioXCell, no. BE0045), for T_Reg TGF-β (3 ng ml⁻¹), anti-IL-4 (10 µg ml⁻¹) and anti-IFN-γ (10 µg ml⁻¹). For T_H17 differentiation, cells were supplemented with 10 ng ml⁻¹ IL-23 (R&D, no. 1887-ML) after 48 h. HB-EGF (50 ng ml⁻¹, Novus Biologicals, no. 35069) was added after 72 h for the rest of the differentiation. After 4 d, production of cytokines was measured by intracellular cytokine staining and subsequent flow cytometry as described above.