The intestine was isolated and fixed with 4% formaldehyde solution, then embedded in OCT. The cryosections were prepared. Then, the sections were permeabilized for 15 min with 0.1% Triton X-100 on ice and blocked in 5% BSA for 1 h at room temperature. The sections were then incubated overnight with the primary antibody at 4 ℃. Finally, the fluorescein-labelled secondary antibodies were applied for 1 h at room temperature. The following antibodies were used: rabbit anti-COX1 (Abcam, 1:200); rabbit anti-COX2 (Abcam, 1:200); mouse anti-PTGES (Santa Cruz, 1:50); rabbit anti-Muc2 (Abcam, 1:300); rabbit anti-Lyz (Abcam, 1:300); rabbit anti-Chga (Abcam, 1:300); rabbit anti-Olfm4 (CST, 1:300).
Rabbit anti chga
Manufactured by Abcam
Rabbit anti-Chga is a primary antibody that specifically binds to and detects the Chromogranin A (Chga) protein. Chga is a widely expressed neuroendocrine secretory protein found in the secretory granules of various neuroendocrine cells and neurons. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and study the Chga protein.
Automatically generated - may contain errors
2 protocols using rabbit anti chga
1
Immunohistochemical Analysis of Intestinal Markers
2
Immunohistochemical Analysis of Intestinal Markers
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The intestine was isolated and fixed with 4% formaldehyde solution, then embedded in OCT. The cryosections were prepared. Then, the sections were permeabilized for 15 min with 0.1% Triton X-100 on ice and blocked in 5% BSA for 1 h at room temperature. The sections were then incubated overnight with the primary antibody at 4 ℃. Finally, the fluorescein-labelled secondary antibodies were applied for 1 h at room temperature. The following antibodies were used: rabbit anti-COX1 (Abcam, 1:200); rabbit anti-COX2 (Abcam, 1:200); mouse anti-PTGES (Santa Cruz, 1:50); rabbit anti-Muc2 (Abcam, 1:300); rabbit anti-Lyz (Abcam, 1:300); rabbit anti-Chga (Abcam, 1:300); rabbit anti-Olfm4 (CST, 1:300).
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