Hdl c

After feeding for 2 or 8 weeks, guinea pigs in the SD group (weighing 340-800 g) and the LD group (weighing 420-1200 g) were anesthetized by 1.5-3.0% isoflurane, and blood samples were obtained from the eyes of guinea pigs (~1 ml per guinea pig) and collected in tubes containing heparin. Plasma was obtained following centrifugation at 1,789 x g for 15 min at 4˚C. Moreover, after resecting the gallbladder, bile was collected and diluted with saline water (1:10), followed by centrifugation at 2,795 x g for 10 min at 4˚C to remove the granular components. Following which, all guinea pigs were euthanized by an overdose of isoflurane. To degrade the bile pigment, bile was illuminated under a daylight lamp for 12 h at 4˚C. The serum levels of T-CHOL, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), as well as the T-CHOL levels in bile were measured using an automatic biochemical analyser (Hitachi, Ltd.) with the corresponding reagents (T-CHOL, cat. no. SNM226; HDL-C, cat. no. SNM224; LDL-C, cat. no. SNM222; all purchased from Beijing Biolab Science & Technology Co., Ltd.). The concentration of CCK8 in serum was measured using an ELISA kit (cat. no. abx150409; Abbexa Ltd.) according to the manufacturer's protocol.