Cells treated with different treatments were incubated with 0.5% Triton X-100 for 1–5 min and were then washed with PBS. The cells were then blocked with 10% goat serum for 15 min at 37 °C and incubated with primary antibodies against MCM7 (A11325, Abclonal, Wuhan, China), p21 (27296-1-AP, Proteintech, Wuhan, China), and p16 (A0262, Abclonal) diluted in PBS overnight at 4 °C. The next day, the cells were incubated with Alexa Fluor 488-conjugated or Alexa Fluor 594-conjugated secondary antibodies (Abcam, MA, USA) and were then stained with DAPI. Finally, images were acquired using a fluorescence microscope (Carl Zeiss, Jena, Germany).
Alexa fluor 488 conjugated or alexa fluor 594 conjugated secondary antibodies
Manufactured by Abcam
Sourced in United States
Alexa Fluor 488-conjugated or Alexa Fluor 594-conjugated secondary antibodies are fluorescent-dye-labeled antibodies used to detect and visualize target proteins in various biological applications, such as immunofluorescence, flow cytometry, and Western blotting. The Alexa Fluor dyes provide bright, photostable fluorescence signals.
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Lab products found in correlation
2 protocols using alexa fluor 488 conjugated or alexa fluor 594 conjugated secondary antibodies
1
Immunofluorescence Analysis of Cell Cycle Markers
2
Immunofluorescent Labeling of TREM1 and CD163
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Sections (5 μm) were cut from paraffin embedded tissues and incubated with primary antibodies against TREM1 (Abcam, ab225861, 1:500) and CD163 (Origene technologies, TA506380, 1:100) at 4°C overnight and then incubated with Alexa Fluor 488-conjugated or Alexa Fluor 594-conjugated secondary antibodies (Abcam, 1:1000) for 2 h. DAPI (Beyotime, 1:1000) was used to stain the nuclei. The immunofluorescent signals were detected by fluorescence microscopy (Leica DMi8; Leica Microsystems).
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