Overnight bacterial enrichment was inoculated into semisolid medium Rappaport–Vassiliadis (HiMedia Laboratories, Pennsylvania), and incubated for 48 h at 43 °C. Samples in which a whitish growing halo was observed were used for PCR analysis using primers for virulence marker invA (Rahn et al. 1992 (link)). Reference strains Salmonella enterica Serotype Typhimurium DT104 was used as positive control. Negative controls (tri-distilled water) were included in all PCR runs.
Rappaport vassiliadis
Manufactured by HiMedia
Sourced in India
The Rappaport–Vassiliadis (RV) is a culture medium used for the selective isolation and enrichment of Salmonella species from clinical and food samples. It is designed to inhibit the growth of other bacteria while promoting the growth of Salmonella.
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Lab products found in correlation
2 protocols using rappaport vassiliadis
1
Salmonella enterica Serotyping Protocol
2
Salmonella Enrichment and Isolation
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The pre-enriched BPW samples were incubated at 37°C for 18–24 h. Then 100μl mixtures was transferred to 10 ml Rappaport-Vassiliadis (HiMedia, India) medium and incubated at higher temperature 41.5°C for 18–24 h, to provide Salmonella with an advantage over most competitive organisms. Additionally; 1 ml of the pre-enrichment broth was transferred into a tube containing 10 ml of Muller-Kauffmann tetrathionate broth (HiMedia, India) and were incubated at 37 °C for 24 h (ISO-6579, 2002 ).
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