Acetyl tubulin

Manufactured by Cell Signaling Technology

Sourced in United States

Acetyl-tubulin is a post-translationally modified form of the tubulin protein, where the lysine residues are acetylated. This modification is associated with the stabilization of microtubules, which are key structural components of the cytoskeleton in eukaryotic cells.

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Close spelling variants of this product

Anti-acetyl-α-tubulin Acetyl-α-tubulin antibody Acetyl‐α‐Tubulin (Lys40) (D20G3) Acetyl-α-tubulin Monoclonal rabbit anti-acetyl-α-tubulin antibody Acetyl-alpha Tubulin Acetyl α-Tubulin (Lys40) Rabbit anti-acetyl-α-tubulin Rabbit monoclonal anti-Acetyl-α-Tubulin Anti-acetyl-α-tubulin (Lys40) (D20G3) XP Rabbit mAb

3 protocols using acetyl tubulin

Antibody Generation and Validation for Acetylation-Specific HMGN2

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Rabbit polyclonal ac-K2-HMGN2 antibody was made by New England Peptides. The antibody was raised against an acetylated N-terminal HMGN2 peptide (P(KAc)RKAEGDAC), followed by affinity purification. To ensure specificity, a blocking peptide (P(KAc)RKAEGDAC) and a non-acetyl blocking peptide (PKRKAEGDAC) were used to ensure acetyl-specific interaction. Antibodies used for western blotting; Tubulin (Invitrogen, 322500, 1:2000); HDAC6 (Abcam, ab47181, 1:500); HMGN2 (Millipore, 07-252, 1:500; Cell Signaling, #9437, 1:2000) pan-acetyl lysine (Millipore, AB3879, 1:500); CISH (Santa Cruz, sc-15344, 1:500); CyclinD1 (Santa Cruz, sc-718, 1:500); CEBPβ (Santa Cruz, sc-150, 1:500); PARP/Cleaved PARP (Cell Signaling, #9542, 1:500); ac-K2-HMGN2 (1:50); acetyl-α-Tubulin (Cell Signaling, #5335, 1:500). Secondary antibody was used at a dilution of 1:1000 for all antibodies except Tubulin, which was 1:2000. Antibodies used for IHC: CyclinD1 (Santa Cruz, sc-718, 1:1500); acetyl-Tubulin (Cell Signaling, D20G3, 1:100); ac-K2-HMGN2 (1:500).

Medler T.R., Craig J.M., Fiorillo A.A., Feeney Y.B., Harrell J.C, & Clevenger C.V. (2016). HDAC6 Deacetylates HMGN2 to Regulate Stat5a Activity and Breast Cancer Growth. Molecular cancer research : MCR, 14(10), 994-1008.

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Western Blot Analysis of KSHV Proteins

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The expression of the proteins of interest was detected by Western Blot using protein-specific antibodies as described previously [44 (link)]. Immuno-reactive bands were identified using a Bio-rad Clarity Max Western ECL Substrate kit, and visualized by Bio-rad Chemi Doc Imaging System. Anti-LANA antibody was purchased from Advanced Biotechnologies Inc (Eldersburg, MD, USA). Antibodies for KSHV RTA and ORF54 were purchased from Helmholtz-Munich, Germany and anti-ORF62 antibody was purchased from Novus Biologicals (Centennial, CO, USA). Antibodies for c-Myc, H3K9Ac, H4K9Ac, cleaved Caspase3, cleaved PARP, p-Rb, p-ATM, acetyl-tubulin and p21 were obtained from Cell Signaling Technology (Danvers, MA, USA).

Chen J., Wang Z., Phuc T., Xu Z., Yang D., Chen Z., Lin Z., Kendrick S., Dai L., Li H.Y, & Qin Z. (2023). Oncolytic strategy using new bifunctional HDACs/BRD4 inhibitors against virus-associated lymphomas. PLOS Pathogens, 19(1), e1011089.

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Antibody Sources and Autophagy Assays

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Antibodies: Antibodies were obtained from the following vendors: SIRT2 (Cell signaling Technology, Danvers, MA, USA D4050), LC3b-II, (Cell signaling Technology E5Q2K), p62 (Abnova, Taipei, Taiwan, 2C11), beclin-1 (Cell signaling, D40C5), total tubulin (Sigma, St. Louis, MO, USA T6199), acetyl tubulin (Cell signaling, D20G3), GAPDH (Santa Cruz Biotechnology, Heidelberg, Germany, sc-322336C5); anti-mouse IgG, HRP-linked antibody (Cell signaling, 7076), anti-rabbit IgG, HRP-linked antibody (Cell signaling, 7074)) Alexa Fluor 488 and 555 from Invitrogen (Carlsbad, CA, USA), biotinylated human SIRT2 antibody (R&D system, Minneapolis, MN, USA, BAF4358). Chemicals were purchased from the following vendors: AK-7 from TOCRIS Bioscience (Minneapolis, MN, USA), Chloroquine diphosphate (P36239, Life technologies, Carlsbad, CA, USA), rapamycin, fatty-acid-free bovine serum albumin, stearic acid and lipopolysaccharide (LPS) from Sigma-Aldrich (St. Louis, MO, USA). TNF-α and IL6 ELISA kit was obtained from BioLegend (San Diego, CA, USA), and an autophagy flux assay kit was purchased from Invitrogen (Carlsbad, CA, USA).

Roychowdhury S., Gandhirajan A., Kibler C., Wang X, & Vachharajani V. (2021). Sirtuin 2 Dysregulates Autophagy in High-Fat-Exposed Immune-Tolerant Macrophages. Cells, 10(4), 731.

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