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Ct26 colon carcinoma cell line

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The CT26 colon carcinoma cell line is a well-established in vitro model derived from a chemically-induced murine colon adenocarcinoma. This cell line is commonly used for cancer research, particularly in the study of colon cancer biology and evaluation of potential therapeutic agents.

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2 protocols using ct26 colon carcinoma cell line

1

Murine Melanoma and Colon Carcinoma Cell Culture

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The murine B16-F10 melanoma cell line and the CT26 colon carcinoma cell line were purchased from the ATCC and cultured in DMEM (Thermo Fisher Scientific, San Jose, CA, USA) supplemented with 10% fetal calf serum (FCS), 100 units/mL penicillin, and 100 μg/mL streptomycin (Gibco, Grand Island, NY, USA). Female C57BL/6 and BALB/c mice were purchased from Slac Laboratory Animal (Shanghai, China) and housed in the Shanghai Jiao Tong University Laboratory Animal Center under specific pathogen-free (SPF) conditions. The mouse experiments were carried out following protocols approved by the laboratory animal center of Shanghai Jiao Tong University.
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2

Photothermal Therapy of Tumor Cells

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4T1 murine breast cancer cell line and CT26 colon carcinoma cell line were both purchased from ATCC (Manassas, VA, EISA) , bone marrow macrophages and DCs were harvested from BALB/c mice, all the cells were cultured following standard protocol. After the co-culturation with different nano-materials, tumor cells were exposed to light (0.75 W/cm2 @5 min) that delivered using an optical fiber with a diffusion micro-lens, from an 805-nm semiconductor laser (AngioDynamics, Inc, NY, USA). Treated cells were harvested for cell death assay, ELISA assay, or flow cytometry assay.
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