The yeast S. cerevisiae strain EBY100 (Invitrogen, Carlsbad, CA) was used for mSEA surface display. The E. coli strains (Invitrogen) Mach1 and BL21 (DE3) were used for recombinant DNA manipulation and protein expression, respectively. All recombinant yeast and E. coli strains are summarized in Supplementary Table 1 . C. thermocellum DSM1237, C. cellulovorans, C. cellulolyticum and R. flavefaciens were purchased from ATCC (Manassas, VA) and cultured anaerobically following ATCC protocols. Recombinant EBY100 cells were cultured using SC-Trp medium: 1.67 g/L yeast nitrogen base without amino acids, 5 g/L ammonium sulfate (Difco Laboratories, Detroit, MI), 20 g/L glucose, 15 g/L adenine hemisulfate, and 0.64 g/L complete supplement mixture without tryptophan (MP Biomedicals, Solon, OH). Induction of aScaf display on yeast surface was performed in YPG media (1% yeast extract, 2% peptone, 2% galactose). E. coli was cultured in Luria-Bertani (LB) medium containing 50 μg/mL kanamycin. Unless otherwise indicated, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
C thermocellum dsm1237
Manufactured by American Type Culture Collection
C. thermocellum DSM1237 is a thermophilic anaerobic bacterium. It is capable of efficient degradation of cellulosic materials through the production of a complex cellulolytic enzyme system.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium sulfate, by BD (2 mentions)
Mach1, by Thermo Fisher Scientific (2 mentions)
Recombinant eby100, by BD (2 mentions)
Adenine hemisulfate, by MP Biomedicals (2 mentions)
Complete supplement mixture without tryptophan, by MP Biomedicals (2 mentions)
C cellulovorans, by American Type Culture Collection (2 mentions)
C cellulolyticum, by American Type Culture Collection (2 mentions)
R flavefaciens, by American Type Culture Collection (2 mentions)
Eby100, by Thermo Fisher Scientific (2 mentions)
2 protocols using c thermocellum dsm1237
1
Yeast and Bacterial Strains for Protein Expression
2
Yeast and Bacterial Strains for Protein Expression
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The yeast S. cerevisiae strain EBY100 (Invitrogen, Carlsbad, CA) was used for mSEA surface display. The E. coli strains (Invitrogen) Mach1 and BL21 (DE3) were used for recombinant DNA manipulation and protein expression, respectively. All recombinant yeast and E. coli strains are summarized in Supplementary Table 1 . C. thermocellum DSM1237, C. cellulovorans, C. cellulolyticum and R. flavefaciens were purchased from ATCC (Manassas, VA) and cultured anaerobically following ATCC protocols. Recombinant EBY100 cells were cultured using SC-Trp medium: 1.67 g/L yeast nitrogen base without amino acids, 5 g/L ammonium sulfate (Difco Laboratories, Detroit, MI), 20 g/L glucose, 15 g/L adenine hemisulfate, and 0.64 g/L complete supplement mixture without tryptophan (MP Biomedicals, Solon, OH). Induction of aScaf display on yeast surface was performed in YPG media (1% yeast extract, 2% peptone, 2% galactose). E. coli was cultured in Luria-Bertani (LB) medium containing 50 μg/mL kanamycin. Unless otherwise indicated, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
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