Goat anti pml n 19

Rabbit anti-PARP-1 (H-250), anti-caspase-3 (H-277) and anti-POT1 (H-200) (Santa Cruz, CA, USA); goat anti-PML (N-19) (Santa Cruz); mouse anti-p53 (DO-1), anti-Cyclin B1 (GNS1), anti-N-myc (B8.4.B) and anti-TRF2 (4A794) (Santa Cruz); rabbit anti-PARP-9, anti-PML, anti-c-Myc (Y69); mouse anti-Rad50 (13B3/2C6), anti-Mre11 (12D7) and anti-NBS1 (NBS1-501) (Abcam, Cambridge, MA, USA); rabbit anti-phospho-histone H2A.X (20E3), anti-p21 (12D1) and anti-Rad50 (Cell Signaling, MA, USA); mouse anti-TRF2 (Cell Signaling); mouse anti-phospho-histone H2A.X (Millipore); rabbit anti-MAD1 (Gene Tex, CA, USA), rabbit anti-NBS1(Novus, Cambridge, UK) and anti-beta actin (AC-15) (Abcam) antibodies were used.

Rabbit anti-PARP-1 (H-250), anti-caspase-3 (H-277), goat anti-PML (N-19) (Santa Cruz), mouse anti-p53 (DO-1 and Fl-393; pan-p53 antibodies recognizing wild type as well as mutant p53 epitopes) (sc-126, Santa Cruz) [49 (link)], Y5-detecting mutant p53 only (ab32049, Abcam); rabbit anti-PARP-9, anti-p21^WAF-1 (12D1) (Cell Signaling Technology) antibodies were used. HP1γ (clone 4252), Phospho p53 (Ser-15) (clone 9284) and anti-53BP1 (BD Transduction Laboratories) were purchased from Millipore and Cell signaling, respectively.