VirNA-mEGFP WR and viRNA-mEGFP MVA complexes were prepared as described in previous section. In addition, both viral strains (WR and MVA) alone or complexed with only lipofectamine were used as controls. For measuring the zeta potential of different complexes, each sample was diluted to a final volume of 700 μL with sterile Milli-Q water adjusted to pH 7.4 and transferred to a DTS1070 disposable capillary cell (Malvern, Worcestershire, UK) for zeta potential measurements. All measurements were performed at 25°C with a Zetasizer Nano ZS (Malvern).
Dts1070 disposable capillary cell
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The DTS1070 is a disposable capillary cell designed for use with Malvern Panalytical's particle characterization instruments. It provides a contained and controlled environment for sample analysis. The capillary cell features a transparent quartz tube that allows for optical measurements. This product is intended to facilitate efficient and reproducible sample analysis.
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Lab products found in correlation
2 protocols using dts1070 disposable capillary cell
1
Measuring Zeta Potential of Viral Complexes
2
Zeta Potential Measurement of Purified Virions
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The purified virions were incubated for 5 minutes and overnight in 1 mM Tris buffer pH 8 and pH 4.5. The virus samples were then transferred to a DTS1070 disposable capillary cell (Malvern, Malvern, Worcestershire, UK) for zeta potential measurements. The measurements were performed at 25°C with a Zetasizer Nano-ZS (Malvern).
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