Log In Sign up
The largest database of trusted experimental protocols

Mirna and mrna primer assays

Manufactured by Qiagen
Visit Supplier

The MiRNA and mRNA primer assays are lab equipment designed for the detection and analysis of microRNA (miRNA) and messenger RNA (mRNA) molecules. These assays provide a standardized and efficient method for the quantification of specific miRNA and mRNA targets in a variety of sample types. The core function of these primer assays is to facilitate the accurate and reproducible measurement of miRNA and mRNA expression levels through real-time PCR technology.

Automatically generated - may contain errors

Lab products found in correlation

Quantifast sybr green rt pcr kit, by Qiagen (2 mentions) Miscript sybr green pcr kit, by Bio-Rad (2 mentions) Miscript 2 rt kit, by Qiagen (2 mentions) Cfx connect real time pcr detection system, by Bio-Rad (2 mentions)

2 protocols using mirna and mrna primer assays

1

Quantitative Analysis of mRNA and miRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was collected using TRIzol® according to standard protocol: https://tools.thermofisher.com/content/sfs/manuals/trizol_reagent.pdf. RT-PCR was performed according to the QIAGEN provided protocol. QIAGEN SYBR® Green QuantiFast RT-PCR kit and protocol was utilized for quantification of mRNA, and QIAGEN miScript II RT Kit and protocol along with QIAGEN miScript SYBR® Green PCR Kit and protocol was utilized for quantification of miRNA with the Bio-Rad CFX Connect™ Real-Time PCR Detection System. miRNA and mRNA primer assays from QIAGEN were utilized. mRNA was normalized to GAPDH and miRNA was normalized to RNU6. Exosomal RNA was collected with the ExoQuick-TC™ reagent and TRIzol® RNA isolation method. Exosome pellets were incubated in TRIzol® for 1–2 hours prior to the isolation procedure. See exosome and RNA collection methods above.
Richards K.E., Zeleniak A.E., Fishel M.L., Wu J., Littlepage L.E, & Hill R. (2016). CANCER-ASSOCIATED FIBROBLAST EXOSOMES REGULATE SURVIVAL AND PROLIFERATION OF PANCREATIC CANCER CELLS. Oncogene, 36(13), 1770-1778.
+ Open protocol
+ Expand
2

Quantitative Analysis of mRNA and miRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was collected using TRIzol® according to standard protocol: https://tools.thermofisher.com/content/sfs/manuals/trizol_reagent.pdf. RT-PCR was performed according to the QIAGEN provided protocol. QIAGEN SYBR® Green QuantiFast RT-PCR kit and protocol was utilized for quantification of mRNA, and QIAGEN miScript II RT Kit and protocol along with QIAGEN miScript SYBR® Green PCR Kit and protocol was utilized for quantification of miRNA with the Bio-Rad CFX Connect™ Real-Time PCR Detection System. miRNA and mRNA primer assays from QIAGEN were utilized. mRNA was normalized to GAPDH and miRNA was normalized to RNU6. Exosomal RNA was collected with the ExoQuick-TC™ reagent and TRIzol® RNA isolation method. Exosome pellets were incubated in TRIzol® for 1–2 hours prior to the isolation procedure. See exosome and RNA collection methods above.
Richards K.E., Zeleniak A.E., Fishel M.L., Wu J., Littlepage L.E, & Hill R. (2016). CANCER-ASSOCIATED FIBROBLAST EXOSOMES REGULATE SURVIVAL AND PROLIFERATION OF PANCREATIC CANCER CELLS. Oncogene, 36(13), 1770-1778.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!