For brain region homogenate, measurement of tumor necrosis factor alpha (TNF-α; proinflammatory cytokine) was estimated using commercially available rat TNF-α enzyme-linked immunosorbent assay (ELISA) Kit (Wuhan USCN Business Co., Ltd., Wuhan, China) following the manufacturer's protocol. For the serum, quantitative measurement of TNF-α was estimated using commercially available rat TNF-α platinum ELISA Kit (eBioscience, San Diego, USA) following the manufacturer's protocol.
Rat tnf α platinum elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Rat TNF-α platinum ELISA Kit is a laboratory assay designed to quantitatively measure the level of Tumor Necrosis Factor alpha (TNF-α) in rat biological samples. It employs the quantitative sandwich enzyme immunoassay technique.
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3 protocols using rat tnf α platinum elisa kit
1
Quantification of Brain and Serum TNF-α
2
Quantifying Inflammatory Markers and Cell Viability
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All chemicals were, unless otherwise stated, of analytical grade and obtained from Sigma-Aldrich (Madison, WI, USA). The Rat TNFα Platinum ELISA kit was obtained from eBioscience (San Diego, CA, USA), Rat Albumin ELISA Quantitation kit was from Bethyl Laboratories (Montgomery, TX, USA). Kit for measurement of lactate dehydrogenase activity was obtained from DiaSys (Holzheim, Germany), substrate for WST-1 test was obtained from Roche (Penzberg, Germany). Fluorescence probe dichlorodihydrofluorescein diacetate (DCFDA) was obtained from Life Technologies (Carlsbad, CA, USA).
3
Quantifying Lung TNF-α by ELISA
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The levels of TNF-α in the supernatants of the lung homogenate and BALF were assayed using rat TNF-α Platinum ELISA kit (eBioscience, San Diego, CA, USA) according to the manufacturer's instructions. The concentrations of TNF-α in samples were determined from a 5-parameter fit of the standard curve constructed using a standard provided with the kit.
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